BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17939

Title: a protein from Haloferax volcanii

Authors: Zhang, Wen; Liao, Shanhui; Fan, Kai

Citation: Liao, Shanhui; Zhang, Wen; Fan, Kai; Tu, Xiaoming. "structure of a protein from Haloferax volcanii"  Not known ., .-..

Assembly members:
a protein from Haloferax volcanii, polymer, 132 residues, 14231.962 Da.

Natural source:   Common Name: Haloferax volcanii   Taxonomy ID: 2246   Superkingdom: Archaea   Kingdom: not available   Genus/species: Haloferax volcanii

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
a protein from Haloferax volcanii: MNVTVEVVGEETSEVAVDDD GTYADLVRAVDLSPHEVTVL VDGRPVPEDQSVEVDRVKVL RLIKGGMNVTVEVVGEETSE VAVDDDGTYADLVRAVDLSP HEVTVLVDGRPVPEDQSVEV DRVKVLRLIKGG

Data sets:
Data typeCount
13C chemical shifts98
15N chemical shifts55
1H chemical shifts339

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1a protein from Haloferax volcanii1

Entities:

Entity 1, a protein from Haloferax volcanii 132 residues - 14231.962 Da.

1   METASNVALTHRVALGLUVALVALGLYGLU
2   GLUTHRSERGLUVALALAVALASPASPASP
3   GLYTHRTYRALAASPLEUVALARGALAVAL
4   ASPLEUSERPROHISGLUVALTHRVALLEU
5   VALASPGLYARGPROVALPROGLUASPGLN
6   SERVALGLUVALASPARGVALLYSVALLEU
7   ARGLEUILELYSGLYGLYMETASNVALTHR
8   VALGLUVALVALGLYGLUGLUTHRSERGLU
9   VALALAVALASPASPASPGLYTHRTYRALA
10   ASPLEUVALARGALAVALASPLEUSERPRO
11   HISGLUVALTHRVALLEUVALASPGLYARG
12   PROVALPROGLUASPGLNSERVALGLUVAL
13   ASPARGVALLYSVALLEUARGLEUILELYS
14   GLYGLY

Samples:

sample_1: a protein from Haloferax volcanii, [15N;13C], 0.6 mM; sodium phosphate 20 mM; sodium chloride 100 mM; H2O 90%; D2O 10%

sample_2: a protein from Haloferax volcanii, [15N;13C], 0.6 mM; sodium phosphate 20 mM; sodium chloride 100 mM; D2O 100%

sample_conditions_1: ionic strength: 0.12 M; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift calculation

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 17204
PDB
GB ADE03392 AKU06380 ELK50451 ELY23894 ELZ70132
REF WP_004045316 WP_004065555 WP_008609462
SP D4GZE7