BMRB Entry 17908

Title:
Solution structure Analysis of the ImKTx104
Deposition date:
2011-09-01
Original release date:
2012-07-23
Authors:
Zeng, D.; Jiang, L.
Citation:

Citation: Chen, Zong-Yun; Zeng, Dan-Yun; Hu, You-Tian; He, Ya-Wen; Pan, Na; Ding, Jiu-Ping; Cao, Zhi-Jian; Liu, Mai-Li; Li, Wen-Xin; Yi, Hong; Jiang, Ling; Wu, Ying-Liang. "Structural and functional diversity of acidic scorpion potassium channel toxins"  PLoS One 7, e35154-e35154 (2012).
PubMed: 22511981

Assembly members:

Assembly members:
entity_1, polymer, 27 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Chinese swimming scorpion   Taxonomy ID: 172552   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Lychas mucronatus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEM-T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ACVTHEDCTLLCYDTIGTCV DGKCKCM

Data sets:
Data typeCount
1H chemical shifts162

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Acidic Scorpion Potassium Channel Toxins1

Entities:

Entity 1, Acidic Scorpion Potassium Channel Toxins 27 residues - Formula weight is not available

1   ALACYSVALTHRHISGLUASPCYSTHRLEU
2   LEUCYSTYRASPTHRILEGLYTHRCYSVAL
3   ASPGLYLYSCYSLYSCYSMET

Samples:

sample: entity_1, [U-15N], 2 mM; sodium phosphate 20 mM; H2O 90 % v/v; D2O, [U-99% 2H], 10 % v/v

sample_conditions_1: pH: 5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
15N-edited 3D NOESY-HSQCsampleisotropicsample_conditions_1
15N,1H-HSQCsampleisotropicsample_conditions_1
1H,1H-TOCSYsampleisotropicsample_conditions_1
1H,1H-NOESYsampleisotropicsample_conditions_1

Software:

CYANA, Guntert P. - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB ACD11798
SP R4GUQ3
AlphaFold R4GUQ3