BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17881

Title: Dynamics of isolated C domain of calmodulin apo form   PubMed: 20973509

Authors: Wang, Xu; Kleerekoper, Quinn; Putkey, John

Citation: Wang, Xu; Kleerekoper, Quinn; Xiong, Liang-wen; Putkey, John. "Intrinsic disorder of PEP-19 confers unique dynamic properties to apo and calcium calmodulin"  Biochemistry 49, 10287-10297 (2010).

Assembly members:
isolcated_c_domain_of_calmodulin, polymer, 71 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
isolcated_c_domain_of_calmodulin: DTDSEEEIREAFRVFDKDGN GYISAAELRHVMTNLGEKLT DEEVDEMIREADIDGDGQVN YEEFVQMMTAK

Data sets:
Data typeCount
15N chemical shifts71
1H chemical shifts71
heteronuclear NOE values69
T1 relaxation values69
T2 relaxation values69

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1isolcated_c_domain_of_calmodulin1

Entities:

Entity 1, isolcated_c_domain_of_calmodulin 71 residues - Formula weight is not available

1   ASPTHRASPSERGLUGLUGLUILEARGGLU
2   ALAPHEARGVALPHEASPLYSASPGLYASN
3   GLYTYRILESERALAALAGLULEUARGHIS
4   VALMETTHRASNLEUGLYGLULYSLEUTHR
5   ASPGLUGLUVALASPGLUMETILEARGGLU
6   ALAASPILEASPGLYASPGLYGLNVALASN
7   TYRGLUGLUPHEVALGLNMETMETTHRALA
8   LYS

Samples:

apo_C-CaM: isolcated c domain of calmodulin, [U-100% 15N], 0.5 mM; EDTA 10 mM; potassium chloride 100 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
T1apo_C-CaMisotropicsample_conditions_1
T2apo_C-CaMisotropicsample_conditions_1
NOEapo_C-CaMisotropicsample_conditions_1
2D 1H-15N HSQCapo_C-CaMisotropicsample_conditions_1
3D HNCAapo_C-CaMisotropicsample_conditions_1
3D HN(CO)CAapo_C-CaMisotropicsample_conditions_1
3D CBCA(CO)NHapo_C-CaMisotropicsample_conditions_1
3D HNCACBapo_C-CaMisotropicsample_conditions_1
3D HNCOapo_C-CaMisotropicsample_conditions_1
3D 1H-15N NOESYapo_C-CaMisotropicsample_conditions_1
3D 1H-15N TOCSYapo_C-CaMisotropicsample_conditions_1

Software:

FELIX v2004, Accelrys Software Inc. - peak picking, processing

NMR spectrometers:

  • Bruker DPX 600 MHz

Related Database Links:

BMRB 11440 16994 17981 17982 17983 18084 18323 19376 25344
PDB
DBJ BAC39089 BAF45809 BAI66109 BAI66110 BAI66111
EMBL CAB51566
GB AAH10730 AAO17827 AAQ14324 AAX61134 AAY87961
REF XP_001687795 XP_001869425 XP_007442524 XP_009577260 XP_010190430