BMRB Entry 17781

Title:
structure of
Deposition date:
2011-07-14
Original release date:
2011-10-25
Authors:
Sprangers, Remco; Mund, Markus; Neu, Ancilla
Citation:

Citation: Mund, Markus; Neu, Ancilla; Ullmann, Janina; Neu, Ursula; Sprangers, Remco. "Structure of the LSm657 complex: an assembly intermediate of the LSm1-7 and LSm2-8 rings."  J. Mol. Biol. 414, 165-176 (2011).
PubMed: 22001694

Assembly members:

Assembly members:
lsm, polymer, 276 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Schizosaccharomyces pombe   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet

Data sets:
Data typeCount
13C chemical shifts620
15N chemical shifts198
1H chemical shifts198

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1lsm51
2lsm61
3lsm71

Entities:

Entity 1, lsm5 276 residues - Formula weight is not available

1   GLYALAMETSERMETTHRILELEUPROLEU
2   GLULEUILEASPLYSCYSILEGLYSERASN
3   LEUTRPVALILEMETLYSSERGLUARGGLU
4   PHEALAGLYTHRLEUVALGLYPHEASPASP
5   TYRVALASNILEVALLEULYSASPVALTHR
6   GLUTYRASPTHRVALTHRGLYVALTHRGLU
7   LYSHISSERGLUMETLEULEUASNGLYASN
8   GLYMETCYSMETLEUILEPROGLYGLYLYS
9   PROGLUGLYALAMETASPSERSERPROASN
10   GLUPHELEUASNLYSVALILEGLYLYSLYS
11   VALLEUILEARGLEUSERSERGLYVALASP
12   TYRLYSGLYILELEUSERCYSLEUASPGLY
13   TYRMETASNLEUALALEUGLUARGTHRGLU
14   GLUTYRVALASNGLYLYSLYSTHRASNVAL
15   TYRGLYASPALAPHEILEARGGLYASNASN
16   VALLEUTYRVALSERALALEUASPASPGLY
17   ALAMETGLYMETSERSERLEUGLNLYSARG
18   PROGLYPROGLYASNSERSERGLNPROTHR
19   GLUARGPROARGLYSGLUSERILELEUASP
20   LEUSERARGTYRGLNASPGLNARGILEGLN
21   ALATHRPHETHRGLYGLYARGGLNILETHR
22   GLYILELEULYSGLYPHEASPGLNLEUMET
23   ASNLEUVALLEUASPASPVALGLUGLUGLN
24   LEUARGASNPROGLUASPGLYLYSLEUTHR
25   GLYALAILEARGLYSLEUGLYLEUVALVAL
26   VALARGGLYTHRTHRLEUVALLEUILEALA
27   PROMETASPGLYSERGLUGLUILEPROASN
28   PROPHEVALGLNALAGLU

Samples:

sample_1: lsm567, [U-100% 13C; U-100% 15N; U-80% 2H], 1 mM; H2O 95%; D2O 5%; NaPO4 50 mM; NaCl 150 mM; DTT 1 mM; NaN3 0.03%

sample_conditions_1: ionic strength: 250 mM; pH: 7.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks