BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17771

Title: Resonance Assignments of Calmodulin Complexed with the Calmodulin-Binding Domain of Olfactory Nucleotide Gated Ion Channel   PubMed: 22877078

Authors: Deli, Irene; Chyan, Chia Lin

Citation: Irene, Deli; Huang, Jian-Wen; Chung, Tse-Yu; Li, Feng-Yin; Tzen, Jason T-C; Lin, Ta-Hsien; Chyan, Chia-Lin. "Binding orientation and specificity of calmodulin to rat olfactory cyclic nucleotide-gated ion channel."  J. Biomol. Struct. Dyn. 31, 414-425 (2013).

Assembly members:
Calmodulin, polymer, 148 residues, Formula weight is not available
OLFp, polymer, 28 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
OLFp: TPRRGRGGFQRIVRLVGVIR DWANKNFR

Data sets:
Data typeCount
13C chemical shifts1371
15N chemical shifts348
1H chemical shifts2278

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Chain 11
2Chain 22
3Calcium ion3

Entities:

Entity 1, Chain 1 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, Chain 2 28 residues - Formula weight is not available

1   THRPROARGARGGLYARGGLYGLYPHEGLN
2   ARGILEVALARGLEUVALGLYVALILEARG
3   ASPTRPALAASNLYSASNPHEARG

Entity 3, Calcium ion - Ca - 40.078 Da.

1   CA

Samples:

CaM-OLFp: Calmodulin, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; OLFp, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; H2O 90%; D2O 10%

sample_conditions: pH: 6.7; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCCaM-OLFpisotropicsample_conditions
2D 1H-13C HSQCCaM-OLFpisotropicsample_conditions
2D 1H-1H TOCSYCaM-OLFpisotropicsample_conditions
2D DQF-COSYCaM-OLFpisotropicsample_conditions
2D 1H-1H NOESYCaM-OLFpisotropicsample_conditions
3D HNCACaM-OLFpisotropicsample_conditions
3D HN(CO)CACaM-OLFpisotropicsample_conditions
3D HNCOCaM-OLFpisotropicsample_conditions
3D HN(CA)COCaM-OLFpisotropicsample_conditions
3D HNCACBCaM-OLFpisotropicsample_conditions
3D C(CO)NHCaM-OLFpisotropicsample_conditions
3D HBHA(CO)NHCaM-OLFpisotropicsample_conditions
3D H(CCO)NHCaM-OLFpisotropicsample_conditions
3D HCCH-TOCSYCaM-OLFpisotropicsample_conditions
3D HCCH-COSYCaM-OLFpisotropicsample_conditions
3D 1H-15N-edited TOCSY HSQCCaM-OLFpisotropicsample_conditions
3D simultaneous 1H-13C/15N-edited NOESY-HSQCCaM-OLFpisotropicsample_conditions

Software:

AURELIA, Neidig, Geyer, Gorler, Antz, Saffrich, Beneicke, Kalbitzer - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4270 4284 4310
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 CAA39135 CAQ34767
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 AAD41473
PIR JC1305 MCON
PRF 0409298A 0608335A 1614345A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 NP_037060 XP_008756353
SP O02367 O16305 O96081 P02594 P05932 Q00195
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988