BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17662

Title: Solution structure of the long sarafotoxin srtx-m   PubMed: 21889567

Authors: Cordier, Florence; Zorba, Adelajda; Hajj, Marianna; Ducancel, Frederic; Servent, Denis; Delepierre, Muriel

Citation: Mourier, Gilles; Hajj, Mariana; Cordier, Florence; Zorba, Adelajda; Gao, XingHuang; Coskun, Tolga; Herbet, Amaury; Marcon, Elodie; Beau, Fabrice; Delepierre, Muriel; Ducancel, Frederic; Servent, Denis. "Pharmacological and structural characterization of long-sarafotoxins, a new family of endothelin-like peptides: Role of the C-terminus extension."  Biochimie 94, 461-470 (2012).

Assembly members:
srtx-m, polymer, 24 residues, 2910.222 Da.

Natural source:   Common Name: snake   Taxonomy ID: 42165   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Atractaspis microlepidota

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
srtx-m: CSCNDINDKECMYFCHQDVI WDEP

Data sets:
Data typeCount
1H chemical shifts160
coupling constants8

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1srtx-m1

Entities:

Entity 1, srtx-m 24 residues - 2910.222 Da.

1   CYSSERCYSASNASPILEASNASPLYSGLU
2   CYSMETTYRPHECYSHISGLNASPVALILE
3   TRPASPGLUPRO

Samples:

sample_1: srtx-m 1.5 mM; H2O 90%; D2O 10%

sample_25C: ionic strength: 0 M; pH: 5; pressure: 1 atm; temperature: 298 K

sample_35C: ionic strength: 0 M; pH: 5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H COSYsample_1isotropicsample_25C
2D 1H-1H TOCSYsample_1isotropicsample_25C
2D 1H-1H NOESYsample_1isotropicsample_25C
2D 1H-1H TOCSYsample_1isotropicsample_35C
2D 1H-1H NOESYsample_1isotropicsample_35C
2D 1H-1H TOCSYsample_1isotropicsample_25C
2D 1H-1H NOESYsample_1isotropicsample_25C
2D 1H-1H NOESYsample_1isotropicsample_35C

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

ARIA, Linge, O, . - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

PDB 2LDF