BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17656

Title: 1H, 13C and 15N NMR assignments of inactive form of P1 endolysin Lyz   PubMed: 21822941

Authors: Jagga, Zeenia; Kashyap, Maruthi; Bhaba, Krishna Das; Arockiasamy, Arulandu; Bhavesh, Neel Sarovar

Citation: Kashyap, Maruthi; Jagga, Zeenia; Das, Bhaba Krishna; Arockiasamy, Arulandu; Bhavesh, Neel Sarovar. "(1)H, (13)C and (15)N NMR assignments of inactive form of P1 endolysin Lyz."  Biomol. NMR Assignments 6, 87-89 (2012).

Assembly members:
P1_endolysin_Lyz, polymer, 163 residues, Formula weight is not available

Natural source:   Common Name: P1 Bacteriophage   Taxonomy ID: not available   Superkingdom: Viruses   Kingdom: not available   Genus/species: P1 Bacteriophage

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
P1_endolysin_Lyz: RTNQAGLELIGNAEGCRRDP YMCPAGVWTDGIGNTHGVTP GVRKTDQQIAADWEKNILIA ERCINQHFRGKDMPDNAFSA MTSAAFNMGCNSLRTYYSKA RGMRVETSIHKWAQKGEWVN MCNHLPDFVNSNGVPLRGLK IRREKERQLCLTGLVNEHHH HHH

Data sets:
Data typeCount
13C chemical shifts632
15N chemical shifts183
1H chemical shifts789

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P1_endolysin_Lyz1

Entities:

Entity 1, P1_endolysin_Lyz 163 residues - Formula weight is not available

1   ARGTHRASNGLNALAGLYLEUGLULEUILE
2   GLYASNALAGLUGLYCYSARGARGASPPRO
3   TYRMETCYSPROALAGLYVALTRPTHRASP
4   GLYILEGLYASNTHRHISGLYVALTHRPRO
5   GLYVALARGLYSTHRASPGLNGLNILEALA
6   ALAASPTRPGLULYSASNILELEUILEALA
7   GLUARGCYSILEASNGLNHISPHEARGGLY
8   LYSASPMETPROASPASNALAPHESERALA
9   METTHRSERALAALAPHEASNMETGLYCYS
10   ASNSERLEUARGTHRTYRTYRSERLYSALA
11   ARGGLYMETARGVALGLUTHRSERILEHIS
12   LYSTRPALAGLNLYSGLYGLUTRPVALASN
13   METCYSASNHISLEUPROASPPHEVALASN
14   SERASNGLYVALPROLEUARGGLYLEULYS
15   ILEARGARGGLULYSGLUARGGLNLEUCYS
16   LEUTHRGLYLEUVALASNGLUHISHISHIS
17   HISHISHIS

Samples:

sample_1: P1 endolysin Lyz, [U-13C; U-15N], 1 mM; H2O 90%; D2O 10%; Sodium Phosphate 50 mM; NaCl 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 4.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - processing

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 700 MHz

Related Database Links:

BMRB 18253
PDB
DBJ BAI39214
EMBL CAA61013 CCP96781 CDK52642 CDK58555 CDK88832
GB AAD20630 AAQ07493 AAQ13989 AAQ14097 AEJ60171
REF WP_000299977 WP_000676956 WP_001339174 WP_001345478 WP_001372701
SP Q37875