BMRB Entry 17645

Title:
Solution structure of myosin VI lever arm extension
Deposition date:
2011-05-13
Original release date:
2012-08-30
Authors:
Feng, Wei; Yu, Cong; Zhang, Mingjie
Citation:

Citation: Yu, Cong; Lou, Jizhong; Wu, Jingjing; Pan, Lifeng; Feng, Wei; Zhang, Mingjie. "Membrane-induced lever arm expansion allows myosin VI to walk with large and variable step sizes."  J. Biol. Chem. 287, 35021-35035 (2012).
PubMed: 22936804

Assembly members:

Assembly members:
myosin_VI_LAE, polymer, 88 residues, 9961.641 Da.

Natural source:

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts305
15N chemical shifts83
1H chemical shifts637

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1myosin VI lever arm extension1

Entities:

Entity 1, myosin VI lever arm extension 88 residues - 9961.641 Da.

Residues "QGPGS" are from the non-native tag

1   GLNGLYPROGLYSERLEUVALLYSVALGLY
2   THRLEULYSLYSARGLEUASPLYSPHEASN
3   GLUVALVALSERALALEULYSASPGLYLYS
4   PROGLUVALASNARGGLNILELYSASNLEU
5   GLUILESERILEASPALALEUMETALALYS
6   ILELYSSERTHRMETMETTHRARGGLUGLN
7   ILEGLNLYSGLUTYRASPALALEUVALLYS
8   SERSERGLUASPLEULEUSERALALEUGLN
9   LYSLYSLYSGLNGLNGLUGLUGLU

Samples:

sample_1: myosin VI LAE 1.0 mM; D2O 100%; sodium phosphate 50 mM

sample_2: myosin VI LAE, [U-100% 15N], 1.0 mM; H2O 90%; D2O 10%

sample_3: myosin VI LAE, [U-100% 13C; U-100% 15N], 1.0 mM; H20 90%; D20 10%

sample_4: myosin VI LAE, [U-100% 13C; U-100% 15N], 1.0 mM; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D HNCACBsample_3isotropicsample_conditions_1
3D CBCA(CO)NHsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_4isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - data analysis

PIPP, Garrett - data analysis

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 750 MHz

Related Database Links:

PDB
DBJ BAD32219
GB AAB00194 AAI44918 AAI50674 EDL26433 EDL26434
PRF 2204386A
REF NP_001034635 XP_001061392 XP_003508949 XP_004696356 XP_004696357
SP Q64331
AlphaFold Q64331

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks