BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17644

Title: Solution structure of the N-terminal domain of huntingtin (htt17) in presence of DPC micelles   PubMed: 23931318

Authors: Michalek, Matthias; Salnikov, Evgeniy; Werten, Sebastiaan; Bechinger, Burkhard

Citation: Michalek, Matthias; Salnikov, Evgeniy; Bechinger, Burkhard. "Structure and Topology of the Huntingtin 1-17 Membrane Anchor by a Combined Solution and Solid-State NMR Approach"  Biophys. J. 105, 699-710 (2013).

Assembly members:
htt17, polymer, 17 residues, 1977.422 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
htt17: MATLEKLMKAFESLKSF

Data sets:
Data typeCount
1H chemical shifts72

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1htt171

Entities:

Entity 1, htt17 17 residues - 1977.422 Da.

1   METALATHRLEUGLULYSLEUMETLYSALA
2   PHEGLUSERLEULYSSERPHE

Samples:

sample_1: htt17, 15N labeled at PHE11, 0.2-0.3 mM; D2O 10%; sodium phosphate 25 mM; DPC, d38, 100 mM; H2O 90%

sample_conditions_1: ionic strength: 0.025 M; pH: 6.0; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Bhattacharya and Montelione, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific, Koradi, Billeter and Wuthrich, Schwieters, Kuszewski, Tjandra and Clore - chemical shift assignment, data analysis, peak picking, processing, refinement, structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 17642 25146
PDB
DBJ BAA36752 BAA36753 BAH03657
EMBL CAA12281
GB AAA89100 AAB38240 AAC52218 AAI72756 AAR34461
REF NP_001136110 NP_001254674 NP_002102 NP_034544 NP_077333
SP P42858 P42859
TPG DAA28444