BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17565

Title: Unmodified Glycyl-tRNA(UCC) anticodon stem-loop from Bacillus subtilis

Authors: Chang, Andrew; Nikonowicz, Edward

Citation: Chang, Andrew; Nikonowicz, Edward. "Structure solution of three glycyl-tRNA anticodon stem-loops"  Not known ., .-..

Assembly members:
Unmodified Glycyl-tRNA(UCC) anticodon stem-loop, polymer, 17 residues, 5379.274 Da.

Natural source:   Common Name: B. subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: enzymatic semisynthesis   Host organism: Bacillus subtilis

Entity Sequences (FASTA):
Unmodified Glycyl-tRNA(UCC) anticodon stem-loop: GGGACCUUCCAAGUCUC

Data sets:
Data typeCount
13C chemical shifts82
1H chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Unmodified Glycyl-tRNA(UCC) anticodon stem-loop1

Entities:

Entity 1, Unmodified Glycyl-tRNA(UCC) anticodon stem-loop 17 residues - 5379.274 Da.

1   GGGACCUUCC
2   AAGUCUC

Samples:

sample_1: RNA, [U-95% 13C; U-95% 15N], 0.02 – 0.04 mM; D2O 100%; KH2PO4 10 mM; K2HPO4 10 mM; KCl 10 mM; EDTA 0.2 mM

sample_2: RNA0.02 – 0.04 mM; D2O 100%; KH2PO4 10 mM; K2HPO4 10 mM; KCl 10 mM; EDTA 0.2 mM

sample_conditions_1: ionic strength: 10 M; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D HCNsample_1isotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - chemical shift assignment, processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links: