BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17564

Title: Glycyl-tRNA(UCC)1B anticodon stem-loop from Staphylococcus epidermidis

Authors: Chang, Andrew; Nikonowicz, Edward

Citation: Chang, Andrew; Nikonowicz, Edward. "Structure solution of three glycyl-tRNA anticodon stem-loop"  Not known ., .-..

Assembly members:
Glycyl-tRNA(UCC)1B anticodon stem-loop, polymer, 17 residues, 5371.249 Da.

Natural source:   Common Name: S. epidermidis   Taxonomy ID: 1282   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus epidermidis

Experimental source:   Production method: enzymatic semisynthesis   Host organism: Staphylococcus epidermidis

Entity Sequences (FASTA):
Glycyl-tRNA(UCC)1B anticodon stem-loop: GGGACCUUCCCGGUCUC

Data sets:
Data typeCount
13C chemical shifts87
1H chemical shifts107

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Glycyl-tRNA(UCC)1B anticodon stem-loop1

Entities:

Entity 1, Glycyl-tRNA(UCC)1B anticodon stem-loop 17 residues - 5371.249 Da.

1   GGGACCUUCC
2   CGGUCUC

Samples:

sample_1: RNA0.02 – 0.04 mM; D2O 100%; KH2PO4 10 mM; K2HPO4 10 mM; KCl 10 mM; EDTA 0.2 mM; MgCl2 10 mM

sample_2: RNA0.02 – 0.04 mM; H2O 90%; D2O 10%; KH2PO4 10 mM; K2HPO4 10 mM; KCl 10 mM; EDTA 0.2 mM; MgCl2 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D HCNsample_1isotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - chemical shift assignment, processing

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links: