BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17488

Title: Spatial structure of dimeric ErbB3 transmembrane domain

Authors: Mineev, Konstantin; Arseniev, Alexander

Citation: Mineev, Konstantin; Khabibullina, Nelya; Lyukmanova, Ekaterina; Dolgikh, Dmitry; Kirpichnikov, Mikhail; Arseniev, Alexander. "Spatial structure and dimer-monomer equilibrium of the ERBB3 transmembrane domain in DPC micelles"  Biochim. Biophys. Acta ., .-..

Assembly members:
ErbB3tm, polymer, 40 residues, 4703.692 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
ErbB3tm: MGRTHLTMALTVIAGLVVIF MMLGGTFLYWRGRRHHHHHH

Data sets:
Data typeCount
13C chemical shifts177
15N chemical shifts47
1H chemical shifts302

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_21

Entities:

Entity 1, entity_1 40 residues - 4703.692 Da.

1   METGLYARGTHRHISLEUTHRMETALALEU
2   THRVALILEALAGLYLEUVALVALILEPHE
3   METMETLEUGLYGLYTHRPHELEUTYRTRP
4   ARGGLYARGARGHISHISHISHISHISHIS

Samples:

water: ErbB3tm, [U-100% 13C; U-100% 15N], 1 mM; ErbB3tm 1 mM; DPC, [U-99% 2H], 80 mM; sodium azide 1 mM; H2O 90%; D2O 10%

D2O: ErbB3tm, [U-100% 13C; U-100% 15N], 1 mM; ErbB3tm 1 mM; DPC, [U-99% 2H], 80 mM; sodium azide 1 mM; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
3D HNCOwaterisotropicsample_conditions_1
3D HNCAwaterisotropicsample_conditions_1
3D HN(CO)CAwaterisotropicsample_conditions_1
3D 1H-15N NOESYwaterisotropicsample_conditions_1
3D 1H-13C NOESYD2Oisotropicsample_conditions_1
2D 1H-15N HSQCwaterisotropicsample_conditions_1
2D 1H-13C HSQC aliphaticD2Oisotropicsample_conditions_1
2D 1H-13C HSQC aromaticD2Oisotropicsample_conditions_1
3D 15N,13C-filtered, 13C-edited NOESYD2Oisotropicsample_conditions_1
3D 15N,13C-filtered, 13C-edited NOESYwaterisotropicsample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

CARA v1.8.5, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

TOPSPIN v2.1, Bruker Biospin - processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAG62544
GB EAW96883