BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17452

Title: Chemical Shifts of the Pitx2 Homeodomain in complex with a TAAGCT non-consensus DNA site   PubMed: 21818549

Authors: Doerdelmann, Thomas; Kojetin, Douglas; Baird-Titus, Jamie; Rance, Mark

Citation: Doerdelmann, Thomas; Kojetin, Douglas; Baird-Titus, Jamie; Rance, Mark. "(1)H, (13)C and (15)N chemical shift assignments for the human Pitx2 homeodomain in complex with a 22-base hairpin DNA"  Biomol. NMR Assignments 6, 79-81 (2012).

Assembly members:
Pitx2_HD, polymer, 68 residues, Formula weight is not available
TAAGCT_DNA, polymer, 6 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Pitx2_HD: GSQRRQRTHFTSQQLQELEA TFQRNRYPDMSTREEIAVWT NLTEARVRVWFKNRRAKWRK REEFIVTD
TAAGCT_DNA: TAAGCT

Data sets:
Data typeCount
13C chemical shifts237
15N chemical shifts89
1H chemical shifts460

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pitx2HD1
2TAAGCT DNA2

Entities:

Entity 1, Pitx2HD 68 residues - Formula weight is not available

First two amino acids are cloning artifacts; EFIVTD C-terminal tail added for stability.

1   GLYSERGLNARGARGGLNARGTHRHISPHE
2   THRSERGLNGLNLEUGLNGLULEUGLUALA
3   THRPHEGLNARGASNARGTYRPROASPMET
4   SERTHRARGGLUGLUILEALAVALTRPTHR
5   ASNLEUTHRGLUALAARGVALARGVALTRP
6   PHELYSASNARGARGALALYSTRPARGLYS
7   ARGGLUGLUPHEILEVALTHRASP

Entity 2, TAAGCT DNA 6 residues - Formula weight is not available

1   DTDADADGDCDT

Samples:

sample_1: Pitx2HD, [U-98% 13C; U-98% 15N], 1.5 mM; TAAGCT DNA 1.5 mM; sodium phosphate 10 mM; EDTA 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.01 M; pH: 7.0; pressure: 1 atm; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz
  • Varian INOVA 400 MHz

Related Database Links:

BMRB 17145 17147 18015
PDB
DBJ BAA75247 BAA75248 BAB69053 BAD12775 BAF84280
EMBL CAA06696 CAA06697 CAA09455 CAB52283 CAB52284
GB AAB38505 AAB38864 AAB87380 AAC16257 AAC24502
REF NP_000316 NP_001007500 NP_001017227 NP_001035436 NP_001035967
SP O35160 O75364 O93385 P81062 P97474
TPG DAA14908 DAA28868