BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17309

Title: Solution structure of coronaviral stem-loop 2 (SL2)   PubMed: 21382373

Authors: Lee, Chul Won; Li, Lichun; Giedroc, David

Citation: Lee, Chul Won; Li, Lichun; Giedroc, David. "The solution structure of coronaviral stem-loop 2 (SL2) reveals a canonical CUYG tetraloop fold."  FEBS Lett. 585, 1049-1053 (2011).

Assembly members:
SL2, polymer, 16 residues, 135.128 Da.

Natural source:   Common Name: coronavirus   Taxonomy ID: 693996   Superkingdom: virus   Kingdom: not available   Genus/species: Alphacoronavirus Alphacoronavirus_1

Experimental source:   Production method: in vitro transcription   Host organism: Escherichia coli

Entity Sequences (FASTA):
SL2: GAUCUCUUGUAGAUCA

Data sets:
Data typeCount
13C chemical shifts35
1H chemical shifts123
31P chemical shifts4

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1coronavirus stem-loop 21

Entities:

Entity 1, coronavirus stem-loop 2 16 residues - 135.128 Da.

1   GAUCUCUUGU
2   AGAUCA

Samples:

SL2_of_SARS_1: SL20.8 – 2 mM; potassium phosphate 10 mM; DSS 0.1 mM; D2O 100%

SL2_of_SARS_2: SL2, [U-13C; U-15N]-Ura, 0.8 – 2 mM; potassium phosphate 10 mM; DSS 0.1 mM; D2O 10%; H2O 90%

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 298 K

sample_conditions_2: pH: 6.0; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYSL2_of_SARS_1isotropicsample_conditions_1
2D 1H-1H TOCSYSL2_of_SARS_1isotropicsample_conditions_1
2D 1H-13C HSQCSL2_of_SARS_1isotropicsample_conditions_1
3D HCCH-TOCSYSL2_of_SARS_1isotropicsample_conditions_1
2D HNCCCHSL2_of_SARS_1isotropicsample_conditions_1
2D H(CCN)H-TOCSYSL2_of_SARS_1isotropicsample_conditions_1
2D HCN-HMQCSL2_of_SARS_1isotropicsample_conditions_1
2D HCN-TROSYSL2_of_SARS_1isotropicsample_conditions_1
2D HCNCHSL2_of_SARS_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

SPARKY, Goddard - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

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