BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17281

Title: Conformational and membrane interactins studies of antimicrobial peptide Alyteserin-1C   PubMed: 21565166

Authors: Subasinghage, Anuhsa; Hewage, Chandralal; Conlon, Michael

Citation: Subasinghage, Anusha; Conlon, Donal; Hewage, J. Michael. "Conformational and membrane interaction studies of the antimicrobial peptide alyteserin-1c and its analogue [E4K]alyteserin-1c."  Biochim. Biophys. Acta 1808, 1975-1984 (2011).

Assembly members:
Alyteserin-1C, polymer, 23 residues, 2270.737 Da.

Natural source:   Common Name: midwife toad   Taxonomy ID: 8443   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Alytes obstetricans

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Alyteserin-1C: GLKEIFKAGLGSLVKGIAAH VAS

Data sets:
Data typeCount
1H chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Alyteserin-1C1

Entities:

Entity 1, Alyteserin-1C 23 residues - 2270.737 Da.

1   GLYLEULYSGLUILEPHELYSALAGLYLEU
2   GLYSERLEUVALLYSGLYILEALAALAHIS
3   VALALASER

Samples:

sample_1: DHPC 290 mM; H2O 90%; D2O 10%; Alyteserin-1C 1.54 mm

sample_conditions_1: pH: 3.5; pressure: 1 atm; temperature: 307 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.0, Bruker Biospin - collection, data analysis, processing

SPARKY v3.114, Goddard - chemical shift assignment, peak picking

CYANA v2.0, Guntert, Mumenthaler and Wuthrich - structure solution

SYBYL v6.8.1, Tripos - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB