BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17217

Title: Solution Structure of the C-terminal Dimerization Domain of SARS Coronavirus Nucleocapsid Protein Solved by the SAIL-NMR Method   PubMed: 18561946

Authors: Takeda, Mitsuhiro; Chang, Chung-ke; Ikeya, Teppei; Guntert, Peter; Chang, Yuan-hsiang; Hsu, Yen-Ian; Huang, Tai-huang; Kainosho, Masatsune

Citation: Takeda, Mitsuhiro; Chang, Chung-ke; Ikeya, Teppei; Guntert, Peter; Chang, Yuan-hsiang; Hsu, Yen-Ian; Huang, Tai-huang; Kainosho, Masatsune. "Solution Structure of the C-terminal Dimerization Domain of SARS Coronavirus Nucleocapsid Protein Solved by the SAIL-NMR Method"  J. Mol. Biol. 380, 608-622 (2008).

Assembly members:
SARS, polymer, 128 residues, Formula weight is not available
dT10, polymer, 10 residues, Formula weight is not available

Natural source:   Common Name: SARS coronavirus   Taxonomy ID: 227859   Superkingdom: virus   Kingdom: not available   Genus/species: Betacoronavirus Severe acute respiratory syndrome-related coronavirus

Experimental source:   Production method: cell free synthesis   Host organism: Escherichia coli

Entity Sequences (FASTA):
SARS: MHHHHHHAMGTKKSAAEASK KPRQKRTATKQYNVTQAFGR RGPEQTQGNFGDQDLIRQGT DYKHWPQIAQFAPSASAFFG MSRIGMEVTPSGTWLTYHGA IKLDDKDPQFKDNVILLNKH IDAYKTFP
dT10: XXXXXXXXXX

Data sets:
  • assigned_chemical_shifts
  • binding_constants
Data typeCount
binding constants1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SARS1
2dT102

Entities:

Entity 1, SARS 128 residues - Formula weight is not available

1   METHISHISHISHISHISHISALAMETGLY
2   THRLYSLYSSERALAALAGLUALASERLYS
3   LYSPROARGGLNLYSARGTHRALATHRLYS
4   GLNTYRASNVALTHRGLNALAPHEGLYARG
5   ARGGLYPROGLUGLNTHRGLNGLYASNPHE
6   GLYASPGLNASPLEUILEARGGLNGLYTHR
7   ASPTYRLYSHISTRPPROGLNILEALAGLN
8   PHEALAPROSERALASERALAPHEPHEGLY
9   METSERARGILEGLYMETGLUVALTHRPRO
10   SERGLYTHRTRPLEUTHRTYRHISGLYALA
11   ILELYSLEUASPASPLYSASPPROGLNPHE
12   LYSASPASNVALILELEULEUASNLYSHIS
13   ILEASPALATYRLYSTHRPHEPRO

Entity 2, dT10 10 residues - Formula weight is not available

X=5AT

1   5AT5AT5AT5AT5AT5AT5AT5AT5AT5AT

Samples:

sample_1: SARS, [U-15N], 0.5 mM; sodium phosphate 10 mM; sodium chloride 50 mM; EDTA 1 mM; DSS 1 mM; sodium azide 0.01%; dT10 titrated mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.0; pressure: ambient atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - processing

iNMR, Nucleomatica, Italy - processing

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

BMRB 15511
PDB
DBJ BAC81358 BAC81372 BAC81386 BAC81400 BAC81414
GB AAP13445 AAP13814 AAP30037 AAP30714 AAP33707
REF NP_828858
SP P59595 Q3I5I7 Q3LZX4