BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17214

Title: A30P alpha-synuclein fibrils   PubMed: 22334684

Authors: Lemkau, Luisel; Comellas, Gemma; Kloepper, Kathryn; Woods, Wendy; George, Julia; Rienstra, Chad

Citation: Lemkau, Luisel; Comellas, Gemma; Kloepper, Kathryn; Woods, Wendy; George, Julia; Rienstra, Chad. "Mutant protein A30P -synuclein adopts wild-type fibril structure, despite slower fibrillation kinetics."  J. Biol. Chem. 287, 11526-11532 (2012).

Assembly members:
A30P_alpha-synuclein, polymer, 140 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
A30P_alpha-synuclein: MDVFMKGLSKAKEGVVAAAE KTKQGVAEAPGKTKEGVLYV GSKTKEGVVHGVATVAEKTK EQVTNVGGAVVTGVTAVAQK TVEGAGSIAAATGFVKKDQL GKNEEGAPQEGILEDMPVDP DNEAYEMPSEEGYQDYEPEA

Data sets:
Data typeCount
13C chemical shifts204
15N chemical shifts52

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1alpha-synuclein fibrils1

Entities:

Entity 1, alpha-synuclein fibrils 140 residues - Formula weight is not available

1   METASPVALPHEMETLYSGLYLEUSERLYS
2   ALALYSGLUGLYVALVALALAALAALAGLU
3   LYSTHRLYSGLNGLYVALALAGLUALAPRO
4   GLYLYSTHRLYSGLUGLYVALLEUTYRVAL
5   GLYSERLYSTHRLYSGLUGLYVALVALHIS
6   GLYVALALATHRVALALAGLULYSTHRLYS
7   GLUGLNVALTHRASNVALGLYGLYALAVAL
8   VALTHRGLYVALTHRALAVALALAGLNLYS
9   THRVALGLUGLYALAGLYSERILEALAALA
10   ALATHRGLYPHEVALLYSLYSASPGLNLEU
11   GLYLYSASNGLUGLUGLYALAPROGLNGLU
12   GLYILELEUGLUASPMETPROVALASPPRO
13   ASPASNGLUALATYRGLUMETPROSERGLU
14   GLUGLYTYRGLNASPTYRGLUPROGLUALA

Samples:

sample_1: A30P alpha-synuclein fibrils, [U-100% 13C; U-100% 15N], 67%; H2O 100%

sample_2: A30P alpha-synuclein fibrils, [U-100% 13C; U-100% 15N], 67%; H2O 100%

sample_conditions_1: pH: 7.5; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
NCACXsample_1isotropicsample_conditions_1
NCOCXsample_1isotropicsample_conditions_1
CANcoCXsample_2isotropicsample_conditions_1
CANCOsample_1isotropicsample_conditions_1

Software:

SPARKY v3.115, Goddard - chemical shift assignment

NMRPipe v2, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian UnityPlus 600 MHz
  • Varian UnityPlus 600 MHz

Related Database Links:

BMRB 16300 16302 16342 16543 16546 16547 16548 16904 16939 17498 17648 17649 17654 17665 17910 18207 18208 18243 18857 18860 19257 19337 19338 19344 19345 19350 19351 25227 25228
PDB
DBJ BAF82858 BAG73790
EMBL CAG33339 CAG46454
GB AAA16117 AAC02114 AAG30302 AAH13293 AAI08276
REF NP_000336 NP_001009158 NP_001032222 NP_001129014 NP_001139526
SP P37840 P61139 P61140 P61142 P61143