BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 17193

Title: NMR Structure in a Membrane Environment Reveals Putative Amyloidogenic Regions of the SEVI Precursor Peptide PAP248-286   PubMed: 19995078

Authors: Nanga, Ravi Prakash Reddy; Brender, Jeffrey Robert; Vivekanandan, Subramanian; Popovych, Nataliya; Ramamoorthy, Ayyalusamy

Citation: Nanga, Ravi Prakash Reddy; Brender, Jeffrey Robert; Vivekanandan, Subramanian; Popovych, Nataliya; Ramamoorthy, Ayyalusamy. "NMR structure in a membrane environment reveals putative amyloidogenic regions of the SEVI precursor peptide PAP(248-286)."  J. Am. Chem. Soc. 131, 17972-17979 (2009).

Assembly members:
PAP248-286, polymer, 39 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):
PAP248-286: GIHKQKEKSRLQGGVLVNEI LNHMKRATQIPSYKKLIMY

Data sets:
Data typeCount
1H chemical shifts253

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAP248-2861

Entities:

Entity 1, PAP248-286 39 residues - Formula weight is not available

1   GLYILEHISLYSGLNLYSGLULYSSERARG
2   LEUGLNGLYGLYVALLEUVALASNGLUILE
3   LEUASNHISMETLYSARGALATHRGLNILE
4   PROSERTYRLYSLYSLEUILEMETTYR

Samples:

sample_1: PAP248-286 2.5 mM; sodium chloride 120 mM; sodium phosphate 20 mM; SDS, [U-99% 2H], 200 mM; D2O, [U-99% 2H], 10%; H2O 10%

sample_conditions_1: ionic strength: 20 mM; pH: 7.3; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

TOPSPIN v2.16, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 17346 17347 17924 17925 18287
PDB
DBJ BAD89417 BAG62248
EMBL CAA36422 CAA37673
GB AAA60021 AAA60022 AAA69694 AAB60640 AAH07460
REF NP_001090 NP_001127666 NP_001278966 XP_001115549 XP_001148736
SP P15309