BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17139

Title: Structure of ARC92VBD/MED25ACID   PubMed: 21378963

Authors: Milbradt, Alexander; Sun, Zhen-Yu; Selenko, Philipp; Takeuchi, Koh; Naar, Anders; Wagner, Gerhard

Citation: Milbradt, Alexander; Kulkarni, Madhura; Yi, Tingfang; Takeuchi, Koh; Sun, Zhen-Yu; Luna, Rafael; Selenko, Philipp; Naar, Anders; Wagner, Gerhard. "Structure of the VP16 transactivator target in the Mediator"  Nat. Struct. Mol. Biol. 18, 410-415 (2011).

Assembly members:
ARC92VBD, polymer, 166 residues, 37940.359 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
ARC92VBD: GEFGQQSVSNKLLAWSGVLE WQEKPKPASVDANTKLTRSL PCQVYVNHGENLKTEQWPQK LIMQLIPQQLLTTLGPLFRN SRMVQFHFTNKDLESLKGLY RIMGNGFAGCVHFPHTAPCE VRVLMLLYSSKKKIFMGLIP YDQSGFVNGIRQVITNHKQV QQQKLE

Data sets:
Data typeCount
13C chemical shifts584
15N chemical shifts156
1H chemical shifts1052

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ARC92VBD1

Entities:

Entity 1, ARC92VBD 166 residues - 37940.359 Da.

Residues 388-390 represent a cloning site and are not part of the natural sequence

1   GLYGLUPHEGLYGLNGLNSERVALSERASN
2   LYSLEULEUALATRPSERGLYVALLEUGLU
3   TRPGLNGLULYSPROLYSPROALASERVAL
4   ASPALAASNTHRLYSLEUTHRARGSERLEU
5   PROCYSGLNVALTYRVALASNHISGLYGLU
6   ASNLEULYSTHRGLUGLNTRPPROGLNLYS
7   LEUILEMETGLNLEUILEPROGLNGLNLEU
8   LEUTHRTHRLEUGLYPROLEUPHEARGASN
9   SERARGMETVALGLNPHEHISPHETHRASN
10   LYSASPLEUGLUSERLEULYSGLYLEUTYR
11   ARGILEMETGLYASNGLYPHEALAGLYCYS
12   VALHISPHEPROHISTHRALAPROCYSGLU
13   VALARGVALLEUMETLEULEUTYRSERSER
14   LYSLYSLYSILEPHEMETGLYLEUILEPRO
15   TYRASPGLNSERGLYPHEVALASNGLYILE
16   ARGGLNVALILETHRASNHISLYSGLNVAL
17   GLNGLNGLNLYSLEUGLU

Samples:

n15: ARC92VBD, [U-99% 15N], 50 – 1000 uM; H2O 95%; D2O 5%

D20: ARC92VBD 1.0 mM; D2O 100%

n15_c13: ARC92VBD, [U-99% 13C; U-99% 15N], 200-1000 uM; H2O 95%; D2O 5%

n15_c13_d2: ARC92VBD, [U-100% 13C; U-100% 15N; U-80% 2H], 700 uM; H2O 95%; D2O 5%

ILV: ARC92VBD, [ILV-100% 13C1H; U-100% 15N; U-100% 2H], 700 uM; D2O 100%

sample_conditions_1: ionic strength: 150 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCn15isotropicsample_conditions_1
2D 1H-15N HSQCn15isotropicsample_conditions_1
2D 1H-1H NOESYD20isotropicsample_conditions_1
3D 1H-15N NOESYn15isotropicsample_conditions_1
3D 1H-13C NOESYn15_c13isotropicsample_conditions_1
3D HNCOn15_c13_d2isotropicsample_conditions_1
3D HNCAn15_c13_d2isotropicsample_conditions_1
3D HN(CO)CAn15_c13_d2isotropicsample_conditions_1
3D HNCACBn15_c13_d2isotropicsample_conditions_1
3D HCCH-TOCSYn15_c13isotropicsample_conditions_1
2D 1H-13C HSQCn15_c13isotropicsample_conditions_1
4D HMQCNOESYHMQCILVisotropicsample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 500 MHz
  • Bruker Avance 900 MHz
  • Bruker Avance 750 MHz

Related Database Links:

BMRB 17323
PDB
DBJ BAE29135 BAF82149 BAG51583 BAG61008
EMBL CAB66680 CAE84581
GB AAG15589 AAH21333 AAH24312 AAH31138 AAH65297
REF NP_001075914 NP_001163897 NP_083641 NP_112235 XP_001115473
SP A2VE44 Q71SY5 Q8VCB2
TPG DAA19611