BMRB Entry 17118

Title:
Conformation of Pseudoazurin in the 152 kDa Electron Transfer Complex with Nitrite Reductase Determined by Paramagnetic NMR
Deposition date:
2010-08-11
Original release date:
2010-11-10
Authors:
Vlasie, Monica; Fernandez-Busnadiego, Ruben; Prudencio, Miguel; Ubbink, Marcellus
Citation:

Citation: Vlasie, Monica; Fernandez-Busnadiego, Ruben; Prudencio, Miguel; Ubbink, Marcellus. "Conformation of Pseudoazurin in the 152 kDa Electron Transfer Complex with Nitrite Reductase Determined by Paramagnetic NMR"  J. Mol. Biol. 375, 1405-1415 (2008).
PubMed: 18083191

Assembly members:

Assembly members:
PAZ, polymer, 123 residues, Formula weight is not available
NiR, polymer, 376 residues, 65.409 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Alcaligenes faecalis   Taxonomy ID: 511   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Alcaligenes faecalis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Data sets:
  • chemical rates
Data typeCount
binding constants1
kinetic rates1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAZ1
2NiR2
3ZN3

Entities:

Entity 1, PAZ 123 residues - Formula weight is not available

1   GLUASNILEGLUVALHISMETLEUASNLYS
2   GLYALAGLUGLYALAMETVALPHEGLUPRO
3   ALATYRILELYSALAASNPROGLYASPTHR
4   VALTHRPHEILEPROVALASPLYSGLYHIS
5   ASNVALGLUSERILELYSASPMETILEPRO
6   GLUGLYALAGLULYSPHELYSSERLYSILE
7   ASNGLUASNTYRVALLEUTHRVALTHRGLN
8   PROGLYALATYRLEUVALLYSCYSTHRPRO
9   HISTYRALAMETGLYMETILEALALEUILE
10   ALAVALGLYASPSERPROALAASNLEUASP
11   GLNILEVALSERALALYSLYSPROLYSILE
12   VALGLNGLUARGLEUGLULYSVALILEALA
13   SERALALYS

Entity 2, NiR 376 residues - 65.409 Da.

1   METALAGLUGLNMETGLNILESERARGARG
2   THRILELEUALAGLYALAALALEUALAGLY
3   ALALEUALAPROVALLEUALATHRTHRSER
4   ALATRPGLYGLNGLYALAVALARGLYSALA
5   THRALAALAGLUILEALAALALEUPROARG
6   GLNLYSVALGLULEUVALASPPROPROPHE
7   VALHISALAHISSERGLNVALALAGLUGLY
8   GLYPROLYSVALVALGLUPHETHRMETVAL
9   ILEGLUGLULYSLYSILEVALILEASPASP
10   ALAGLYTHRGLUVALHISALAMETALAPHE
11   ASNGLYTHRVALPROGLYPROLEUMETVAL
12   VALHISGLNASPASPTYRLEUGLULEUTHR
13   LEUILEASNPROGLUTHRASNTHRLEUMET
14   HISASNILEASPPHEHISALAALATHRGLY
15   ALALEUGLYGLYGLYGLYLEUTHRGLUILE
16   ASNPROGLYGLULYSTHRILELEUARGPHE
17   LYSALATHRLYSPROGLYVALPHEVALTYR
18   HISCYSALAPROPROGLYMETVALPROTRP
19   HISVALVALSERGLYMETASNGLYALAILE
20   METVALLEUPROARGGLUGLYLEUHISASP
21   GLYLYSGLYLYSALALEUTHRTYRASPLYS
22   ILETYRTYRVALGLYGLUGLNASPPHETYR
23   VALPROARGASPGLUASNGLYLYSTYRLYS
24   LYSTYRGLUALAPROGLYASPALATYRGLU
25   ASPTHRVALLYSVALMETARGTHRLEUTHR
26   PROTHRHISVALVALPHEASNGLYALAVAL
27   GLYALALEUTHRGLYASPLYSALAMETTHR
28   ALAALAVALGLYGLULYSVALLEUILEVAL
29   HISSERGLNALAASNARGASPTHRARGPRO
30   HISLEUILEGLYGLYHISGLYASPTYRVAL
31   TRPALATHRGLYLYSPHEASNTHRPROPRO
32   ASPVALASPGLNGLUTHRTRPPHEILEPRO
33   GLYGLYALAALAGLYALAALAPHETYRTHR
34   PHEGLNGLNPROGLYILETYRALATYRVAL
35   ASNHISASNLEUILEGLUALAPHEGLULEU
36   GLYALAALAALAHISPHELYSVALTHRGLY
37   GLUTRPASNASPASPLEUMETTHRSERVAL
38   LEUALAPROSERGLYTHR

Entity 3, ZN - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: PAZ, [U-15N; U-2H], 250 uM; Zinc 250 mM; sodium phosphate buffer 20 mM; NiR1 – 3 mM; H20 94%; D20 6%

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: ambient atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N-1H TROSYsample_1isotropicsample_conditions_1

Software:

AZARA, Boucher - processing

ANSIG, Kraulis - data analysis

OriginPro v7.5, (OriginLab Corp., Northampton, MA) - data analysis

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
GB AAA21955 ABS15608 AIK42710 EXL05187 KIU70478 AAX10149 ABS17079 AIK41310 EEQ95508 ELT48746
PRF 1204196A
REF WP_010660860 WP_043061611 WP_006466572 WP_010660614 WP_012093660 WP_021584991 WP_036588193
SP P04377 P38501
DBJ BAA02440 BAH28817
AlphaFold P04377 P38501