BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17078

Title: 1H, 15N chemical shift assignments of the imino groups in the base pairs of Escherichia coli tRNALeu (CAG)   PubMed: 20931304

Authors: Hao, Zhan-Xi; Feng, Rui; Wang, En-Duo; Zhu, Guang

Citation: Hao, Zhan-Xi; Feng, Rui; Wang, En-Duo; Zhu, Guang. "(1)H, (15)N chemical shift assignments of the imino groups in the base pairs of Escherichia coli tRNA(Leu) (CAG)."  Biomol. NMR Assignments 5, 71-74 (2011).

Assembly members:
leucine_transfer_ribonucleic_acid, polymer, 87 residues, Formula weight is not available
MG, non-polymer, 24.305 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
leucine_transfer_ribonucleic_acid: GCGAAGGUGGCGGAAXXXGX AGACGCGCUAGCUUCAGXXG XUAGUGUCCUUACGGACGUG GGGGXXCAAGUCCCCCCCCU CGCACCA

Data sets:
Data typeCount
15N chemical shifts32
1H chemical shifts32

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tRNA monomer1
2magnesium ion2

Entities:

Entity 1, tRNA monomer 87 residues - Formula weight is not available

1   GCGAAGGUGG
2   CGGAAH2UH2UOMGGH2U
3   AGACGCGCUA
4   GCUUCAG1MGPSUG
5   PSUUAGUGUCCU
6   UACGGACGUG
7   GGGG5MUPSUCAAG
8   UCCCCCCCCU
9   CGCACCA

Entity 2, magnesium ion - Mg - 24.305 Da.

1   MG

Samples:

15N-EctRNALeu(CAG): tRNALeu, [U-95% 15N], 1 mM; MgCl2 10 mM; NaCl 100 mM; sodium phosphate 10 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N TROSY15N-EctRNALeu(CAG)isotropicsample_conditions_1
2D 1H-1H NOESY15N-EctRNALeu(CAG)isotropicsample_conditions_1
3D 1H-15N NOESY15N-EctRNALeu(CAG)isotropicsample_conditions_1

Software:

VNMRJ, Varian - collection, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

tRNAdb tdbR00000221