BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16947

Title: Backbone structure of the membrane domain of E. coli histidine kinase receptor ArcB   PubMed: 20498088

Authors: Maslennikov, Innokentiy; Klammt, Christian; Hwang, Eunha; Kefala, Georgia; Kwiatkowski, Witek; Jeon, Young Ho; Choe, Senyon

Citation: Maslennikov, Innokentiy; Klammt, Christian; Hwang, Eunha; Kefala, Georgia; Okamura, Mizuki; Esquivies, Luis; Mors, Karsten; Glaubitz, Clemens; Kwiatkowski, Witek; Jeon, Young Ho; Choe, Senyon. "Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis."  Proc. Natl. Acad. Sci. U.S.A. 107, 10902-10907 (2010).

Assembly members:
ArcB, polymer, 115 residues, 8483.369 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free

Entity Sequences (FASTA):
ArcB: MKQIRLLAQYYVDLMMKLGL VRFSMLLALALVVLAIVVQM AVTMVLHGQVESIDVIRSIF FGLLITPWAVYFLSVVVEQL EESRQRLSRLVQKLEEMRER DLSLNVQLKDNIAQL

Data sets:
Data typeCount
13C chemical shifts317
15N chemical shifts108
1H chemical shifts491

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ArcB1

Entities:

Entity 1, ArcB 115 residues - 8483.369 Da.

The membrane domain of ArcB

1   METLYSGLNILEARGLEULEUALAGLNTYR
2   TYRVALASPLEUMETMETLYSLEUGLYLEU
3   VALARGPHESERMETLEULEUALALEUALA
4   LEUVALVALLEUALAILEVALVALGLNMET
5   ALAVALTHRMETVALLEUHISGLYGLNVAL
6   GLUSERILEASPVALILEARGSERILEPHE
7   PHEGLYLEULEUILETHRPROTRPALAVAL
8   TYRPHELEUSERVALVALVALGLUGLNLEU
9   GLUGLUSERARGGLNARGLEUSERARGLEU
10   VALGLNLYSLEUGLUGLUMETARGGLUARG
11   ASPLEUSERLEUASNVALGLNLEULYSASP
12   ASNILEALAGLNLEU

Samples:

sample_EC: DSS 0.5 mM; sodium acetate 20 mM; 1-myristoyl-2-hydroxy-sn-glycero-3-[phospho-rac-(1-glycerol)] 200 mM; sodium chloride 10 mM; Protein, [U-98% 13C; U-98% 15N], 0.5 mM; H2O 95%; D2O 5%

sample_CF: DSS 0.5 mM; 1-myristoyl-2-hydroxy-sn-glycero-3-[phospho-rac-(1-glycerol)] 100 mM; Mes-BisTris 20 mM; Protein, [U-99% 13C; U-99% 15N], 0.3 mM; H2O 95%; D2O 5%

sample_conditions_EC: pH: 5.5; pressure: 1 atm; temperature: 318 K

sample_conditions_CF: pH: 5.5; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_ECisotropicsample_conditions_EC
2D 1H-15N HSQCsample_CFisotropicsample_conditions_CF
3D 1H-15N NOESYsample_ECisotropicsample_conditions_EC
3D 1H-15N NOESYsample_CFisotropicsample_conditions_CF
3D HNCAsample_CFisotropicsample_conditions_CF
3D HNCOsample_CFisotropicsample_conditions_CF
3D HNCACBsample_CFisotropicsample_conditions_CF
3D HN(CO)CAsample_CFisotropicsample_conditions_CF
3D HN(CA)COsample_CFisotropicsample_conditions_CF

Software:

CYANA v1.0.6, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN v2.1, Bruker Biospin - collection

CARA v1.8.4, Keller and Wuthrich - data analysis, peak picking

Molmol v2K.2, Koradi, Billeter and Wuthrich - structure visualization and analysis

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAB37512 BAE77254 BAG79018 BAH65394 BAI27488
EMBL CAA37397 CAD07845 CAP77670 CAQ33543 CAQ90680
GB AAA58012 AAG58344 AAL22197 AAN44715 AAN82410
PIR AG0906
REF NP_312116 NP_457707 NP_462238 NP_709008 WP_000809745
SP P0AEC3 P0AEC4 P58363