BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16868

Title: Solution structure of the aminoterminal domain of E. coli NusA

Authors: Schweimer, Kristian; Jurk, Marcel; Roesch, Paul

Citation: Jurk, Marcel; Schweimer, Kristian; Roesch, Paul. "Solution structure of the aminoterminal domain of E. coli NusA"  Not known ., .-..

Assembly members:
NusA, polymer, 129 residues, 14894.971 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
NusA: MNKEILAVVEAVSNEKALPR EKIFEALESALATATKKKYE QEIDVRVQIDRKSGDFDTFR RWLVVDEVTQPTKEITLEAA RYEDESLNLGDYVEDQIESV TFDRITTQTAKQVIVQKVRE AERAMLVPR

Data sets:
Data typeCount
13C chemical shifts508
15N chemical shifts119
1H chemical shifts868

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NusA1

Entities:

Entity 1, NusA 129 residues - 14894.971 Da.

1   METASNLYSGLUILELEUALAVALVALGLU
2   ALAVALSERASNGLULYSALALEUPROARG
3   GLULYSILEPHEGLUALALEUGLUSERALA
4   LEUALATHRALATHRLYSLYSLYSTYRGLU
5   GLNGLUILEASPVALARGVALGLNILEASP
6   ARGLYSSERGLYASPPHEASPTHRPHEARG
7   ARGTRPLEUVALVALASPGLUVALTHRGLN
8   PROTHRLYSGLUILETHRLEUGLUALAALA
9   ARGTYRGLUASPGLUSERLEUASNLEUGLY
10   ASPTYRVALGLUASPGLNILEGLUSERVAL
11   THRPHEASPARGILETHRTHRGLNTHRALA
12   LYSGLNVALILEVALGLNLYSVALARGGLU
13   ALAGLUARGALAMETLEUVALPROARG

Samples:

sample_1: NusA, [U-98% 13C; U-98% 15N], 0.4 mM; potassium phosphate 10 mM; sodium chloride 50 mM; EDTA 50 uM; H2O 90%; D2O 10%

sample_2: NusA, [U-98% 13C; U-98% 15N], 0.4 mM; potassium phosphate 10 mM; sodium chloride 50 mM; EDTA 50 uM; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D CCH-TOCSYsample_2isotropicsample_conditions_1
3D CCH-NOESYsample_2isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMRView, Johnson, One Moon Scientific - data analysis

xwinnmr, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAB37473 BAE77215 BAG78979 BAI27449 BAI32628
EMBL CAA25200 CAP77631 CAQ33504 CAQ90641 CAR00133
GB AAA57972 AAC76203 AAG58305 AAN44677 AAN82367
REF NP_312077 NP_417638 NP_708970 WP_001031044 WP_001031045
SP P0AFF6 P0AFF7 P0AFF8 P0AFF9