BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16843

Title: Seeing the Invisible: Structures of Excited Protein States by Relaxation Dispersion NMR   PubMed: 24805164

Authors: Vallurupalli, Pramodh; Hansen, Flemming; Kay, Lewis

Citation: Sanchez-Medina, Celia; Sekhar, Ashok; Vallurupalli, Pramodh; Cerminara, Michele; Munoz, Victor; Kay, Lewis. "Probing the Free Energy Landscape of the Fast-Folding gpW Protein by Relaxation Dispersion NMR."  J. Am. Chem. Soc. ., .-. (2014).

Assembly members:
Abp1p, polymer, 62 residues, Formula weight is not available
Ark1p, polymer, 17 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Abp1p: GAMAPWATAEYDYDAAEDNE LTFVENDKIINIEFVDDDWW LGELEKDGSKGLFPSNYVSL GN
Ark1p: KKTKPTPPPKPSHLKPK

Data sets:
Data typeCount
13C chemical shifts99
15N chemical shifts57
1H chemical shifts56

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Abp1p1
2Ark1p2

Entities:

Entity 1, Abp1p 62 residues - Formula weight is not available

Starting Residues GAM are not numbered and not used.

1   GLYALAMETALAPROTRPALATHRALAGLU
2   TYRASPTYRASPALAALAGLUASPASNGLU
3   LEUTHRPHEVALGLUASNASPLYSILEILE
4   ASNILEGLUPHEVALASPASPASPTRPTRP
5   LEUGLYGLULEUGLULYSASPGLYSERLYS
6   GLYLEUPHEPROSERASNTYRVALSERLEU
7   GLYASN

Entity 2, Ark1p 17 residues - Formula weight is not available

1   LYSLYSTHRLYSPROTHRPROPROPROLYS
2   PROSERHISLEULYSPROLYS

Samples:

sample_1: Abp1p, [U-90% 2H; U-100% 15N], 1.5 mM; Ark1p, [U-90% 2H; U-100% 15N], 0.105 mM; Sodium Phosphate 50 mM; NaCl 100 mM; EDTA 1 mM; NaN3 1 mM

sample_2: Abp1p, [U-90% 2H; U-100% 15N], 1.5 mM; Ark1p 0.105 mM; Sodium Phosphate 50 mM; NaCl 100 mM; EDTA 1 mM; NaN3 1 mM

sample_3: Abp1p, [U-90% 2H; U-100% 15N; 13CO labeled], 1.5 mM; Ark1p 0.105 mM; Sodium Phosphate 50 mM; NaCl 100 mM; EDTA 1 mM; NaN3 1 mM

sample_4: Abp1p, [U-90% 2H; U-100% 15N; 13Ca labeled], 1.5 mM; Ark1p 0.105 mM; Sodium Phosphate 50 mM; NaCl 100 mM; EDTA 1 mM; NaN3 1 mM

sample_conditions_1: ionic strength: 0.150 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15N CT-CW,TROSY,anti-TROSY CPMGsample_1anisotropicsample_conditions_1
15N CT-CW,TROSY,anti-TROSY CPMGsample_2anisotropicsample_conditions_1
13CO CT-inphase,TROSY,anti-TROSY CPMGsample_3anisotropicsample_conditions_1
13CA CT-CW,TROSY,anti-TROSY CPMGsample_4anisotropicsample_conditions_1
15N CT-CW CPMGsample_2isotropicsample_conditions_1
13 CO CT CPMGsample_3isotropicsample_conditions_1
13 CA CT-CW CPMGsample_4isotropicsample_conditions_1
1H CT-CW CPMGsample_2isotropicsample_conditions_1

Software:

X-PLOR NIH v2.19, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 18054 18055 18056 18057 18058 18059 18060 18061 18062 18063 18064 18065 18066 18067 18068 18069 18070 18071 18072 18073 18074 18075 18076 18077 18078 25354 26519 17223 18055 18067
PDB
DBJ GAA21988 GAA26063
EMBL CAA36075 CAA42253 CAY78291 CAA95882
GB AHN96147 AHV79349 AHY79755 AJP37506 AJQ31991 AHY77072 EDN62778 EDV12290 EEU04773 EGA56977
PRF 1603360A
REF NP_010012 NP_014378
SP P15891 P53974
TPG DAA07557 DAA10525