BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16790

Title: Solution NMR structure of uncharacterized protein CV0863 from Chromobacterium violaceum. NESG target CvT3. OCSP target CV0863.

Authors: Gutmanas, Aleksandras; Fares, Christophe; Yee, Adelinda; Lemak, Alexander; Semesi, Anthony; Arrowsmith, Cheryl

Citation: Gutmanas, Aleksandras; Fares, Christophe; Yee, Adelinda; Lemak, Alexander; Arrowsmith, Cheryl. "Solution NMR structure of uncharacterized protein CV0863 from Chromobacterium Violaceum"  To Be Published ., .-..

Assembly members:
CV0863, polymer, 111 residues, 12487.9114 Da.

Natural source:   Common Name: Chromobacterium violaceum ATCC 12472   Taxonomy ID: 243365   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Chromobacterium violaceum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CV0863: QGMEVSANELEAASSRMEML QREYSTLRSVQYRSEEGVIV FILANDRELKFRPDDLQATY GATPEQLREIEISPSGLGVY FETLEEDVSLIGLLEGRRGS AKWMAEHPLAS

Data sets:
Data typeCount
13C chemical shifts479
15N chemical shifts109
1H chemical shifts765

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CV08631

Entities:

Entity 1, CV0863 111 residues - 12487.9114 Da.

Cloning artifacts present (first two residues)

1   GLNGLYMETGLUVALSERALAASNGLULEU
2   GLUALAALASERSERARGMETGLUMETLEU
3   GLNARGGLUTYRSERTHRLEUARGSERVAL
4   GLNTYRARGSERGLUGLUGLYVALILEVAL
5   PHEILELEUALAASNASPARGGLULEULYS
6   PHEARGPROASPASPLEUGLNALATHRTYR
7   GLYALATHRPROGLUGLNLEUARGGLUILE
8   GLUILESERPROSERGLYLEUGLYVALTYR
9   PHEGLUTHRLEUGLUGLUASPVALSERLEU
10   ILEGLYLEULEUGLUGLYARGARGGLYSER
11   ALALYSTRPMETALAGLUHISPROLEUALA
12   SER

Samples:

sample_1_1: CV0863, [U-100% 13C; U-100% 15N], 1.0 mM; TRIS 10.0 mM; NaCl 300.0 mM; ZnSO4 10.0 mM; DTT 10.0 mM; benzamidine 1.0 mM; NaN3 0.01%; Roch inhibitor cocktail 1 x

sample_2_1: CV0863, [U-7% 13C; U-100% 15N], 1.0 mM; TRIS 10.0 mM; NaCl 300.0 mM; ZnSO4 10.0 mM; DTT 10.0 mM; benzamidine 1.0 mM; NaN3 0.01%; Roche inhibitor cocktail 1 x

sample_conditions_1: ionic strength: 300.0 mM; pH: 7.0; pressure: 1.0 atm; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
13C-NOESY-HSQC-aliphaticsample_1_1isotropicsample_conditions_1
13C-HSQC-CT-aliphaticsample_1_1isotropicsample_conditions_1
13C-HSQC-aliphaticsample_1_1isotropicsample_conditions_1
13C-HSQC-aromaticsample_1_1isotropicsample_conditions_1
15N-HSQCsample_1_1isotropicsample_conditions_1
13C-NOESY-HSQC-aromaticsample_1_1isotropicsample_conditions_1
15N-NOESY-HSQCsample_1_1isotropicsample_conditions_1
H(C)CH-TOCSY-aliphaticsample_1_1isotropicsample_conditions_1
(H)CCH-TOCSY-aliphaticsample_1_1isotropicsample_conditions_1
H(C)CH-TOCSY-aromaticsample_1_1isotropicsample_conditions_1
(H)CCH-TOCSY-aromaticsample_1_1isotropicsample_conditions_1
T1sample_1_1isotropicsample_conditions_1
T1rhosample_1_1isotropicsample_conditions_1
HNCOsample_1_1isotropicsample_conditions_1
HNCAsample_1_1isotropicsample_conditions_1
CBCA(CO)NHsample_1_1isotropicsample_conditions_1
HBHA(CBCACO)NHsample_1_1isotropicsample_conditions_1
H(CCCO)NH-TOCSYsample_1_1isotropicsample_conditions_1
13C-HSQC-CT-aliphaticsample_2_1isotropicsample_conditions_1

Software:

ABACUS v2.2, A. Lemak - chemical shift assignment

CCPN_Analysis vany, CCPN - data analysis, peak picking

CNS vany, Brunger, Adams,Clore,Gros,Nilges,Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CcpNmr_Entry_Completion_Interface v2.1, PDBe & CCPN - data deposition

MDDGUI, A. Gutmanas - processing

MDDNMR vany, V. Orekhov - processing

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz
  • Bruker AVANCE 800 MHz
  • Bruker AVANCE 800 MHz

Related Database Links:

PDB
GB AAQ58538
REF WP_052278761 WP_052941933