BMRB Entry 16787

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR16787

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Title:
NMR assignments for Thermus thermophilus Rieske protein at pH 5.2 at the reduced state
Deposition date:
2010-03-23
Original release date:
2010-06-15
Authors:
Hsueh, Kuang-Lung; Markley, John
Citation:

Citation: Hsueh, Kuang-Lung; Westler, William; Markley, John. "NMR investigations of the Rieske protein from Thermus thermophilus support a coupled proton and electron transfer mechanism."  J. Am. Chem. Soc. 132, 7908-7918 (2010).
PubMed: 20496909

Assembly members:

Assembly members:
Rieske_protein, polymer, 157 residues, Formula weight is not available
[2Fe-2S], non-polymer, Formula weight is not available

Natural source:

Natural source:   Common Name: Thermus thermophilus   Taxonomy ID: 274   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET17b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rieske_protein: MTPEKEPLKPGDILVYAQGG GEPKPIRLEELKPGDPFVLA YPMDPKTKVVKSGEAKNTLL VARFDPEELAPEVAQHAAEG VVAYSAVCTHLGPIVSQFVA DEEAALCPCPGPVYDLRHGA QVIAGPPPRPVPQLPVRVED GVLVAAGEFLGPVGVQA

Data sets:
Data typeCount
13C chemical shifts335
15N chemical shifts131
1H chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rieske protein1
2[2Fe-2S] cofactor2

Entities:

Entity 1, Rieske protein 157 residues - Formula weight is not available

1   METTHRPROGLULYSGLUPROLEULYSPRO
2   GLYASPILELEUVALTYRALAGLNGLYGLY
3   GLYGLUPROLYSPROILEARGLEUGLUGLU
4   LEULYSPROGLYASPPROPHEVALLEUALA
5   TYRPROMETASPPROLYSTHRLYSVALVAL
6   LYSSERGLYGLUALALYSASNTHRLEULEU
7   VALALAARGPHEASPPROGLUGLULEUALA
8   PROGLUVALALAGLNHISALAALAGLUGLY
9   VALVALALATYRSERALAVALCYSTHRHIS
10   LEUGLYPROILEVALSERGLNPHEVALALA
11   ASPGLUGLUALAALALEUCYSPROCYSPRO
12   GLYPROVALTYRASPLEUARGHISGLYALA
13   GLNVALILEALAGLYPROPROPROARGPRO
14   VALPROGLNLEUPROVALARGVALGLUASP
15   GLYVALLEUVALALAALAGLYGLUPHELEU
16   GLYPROVALGLYVALGLNALA

Entity 2, [2Fe-2S] cofactor - . - Formula weight is not available

1   [2Fe-2S]

Samples:

sample_2_reduced: Thermus thermophilus Rieske protein 5 ± 0.1 mM; TRIS 20 ± 1 mM; sodium phosphate 20 ± 1 mM; boric acid 20 ± 1 mM; dithionite 30 ± 3 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.4 M; pH: 5.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_2_reducedisotropicsample_conditions_1
3D CBCA(CO)NHsample_2_reducedisotropicsample_conditions_1
3D HNCACBsample_2_reducedisotropicsample_conditions_1

Software:

SPARKY v3.114, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16804
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks