BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16752

Title: Mapping the Interacting Regions between Troponins T and C   PubMed: 11473120

Authors: Blumenschein, Tharin; Tripet, Brian; Hodges, Robert; Sykes, Brian

Citation: Blumenschein, Tharin; Tripet, Brian; Hodges, Robert; Sykes, Brian. "Mapping the Interacting Regions between Troponins T and C"  J. Biol. Chem. 276, 36606-36612 (2001).

Assembly members:
TnC, polymer, 161 residues, Formula weight is not available
TnT(160-193), polymer, 34 residues, Formula weight is not available

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
TnC: MDDIYKAAVEQLTEEQKNEF KAAFDIFVLGAEDGCISTKE LGKVMRMLGQNPTPEELQEM IDEVDEDGSGTVDFDEFLVM MVRCMKDDSKGKTEEELSDL FRMFDKNADGYIDLEELKIM LQATGETITEDDIEELMKDG DKNNDGRIDYDEFLEFMKGV E
TnT(160-193): LAKADQKRGKKQTAREMKKK ILAERRKPLNIDHL

Data sets:
  • assigned_chemical_shifts
  • binding_constants
Data typeCount
binding constants4

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TnC1
2TnT(160-193)2

Entities:

Entity 1, TnC 161 residues - Formula weight is not available

1   METASPASPILETYRLYSALAALAVALGLU
2   GLNLEUTHRGLUGLUGLNLYSASNGLUPHE
3   LYSALAALAPHEASPILEPHEVALLEUGLY
4   ALAGLUASPGLYCYSILESERTHRLYSGLU
5   LEUGLYLYSVALMETARGMETLEUGLYGLN
6   ASNPROTHRPROGLUGLULEUGLNGLUMET
7   ILEASPGLUVALASPGLUASPGLYSERGLY
8   THRVALASPPHEASPGLUPHELEUVALMET
9   METVALARGCYSMETLYSASPASPSERLYS
10   GLYLYSTHRGLUGLUGLULEUSERASPLEU
11   PHEARGMETPHEASPLYSASNALAASPGLY
12   TYRILEASPLEUGLUGLULEULYSILEMET
13   LEUGLNALATHRGLYGLUTHRILETHRGLU
14   ASPASPILEGLUGLULEUMETLYSASPGLY
15   ASPLYSASNASNASPGLYARGILEASPTYR
16   ASPGLUPHELEUGLUPHEMETLYSGLYVAL
17   GLU

Entity 2, TnT(160-193) 34 residues - Formula weight is not available

1   LEUALALYSALAASPGLNLYSARGGLYLYS
2   LYSGLNTHRALAARGGLUMETLYSLYSLYS
3   ILELEUALAGLUARGARGLYSPROLEUASN
4   ILEASPHISLEU

Samples:

sample_1: TnC, [U-15N], 0.6 mM; TnT(160-193)21 – 62 mM; potassium chloride 100 mM; imidazole 10 mM; dithiothreitol 20 mM; CaCl2 6 mM; sodium azide 0.03%; 2,2-dimethyl-2-silapentane-5-sulfonic acid 0.2 mM; H2O 90%; D2O 10%

sample_2: TnC, [U-13C; U-15N], 0.9 mM; TnT(160-193) 33 mM; potassium chloride 100 mM; imidazole 10 mM; dithiothreitol 20 mM; CaCl2 6 mM; sodium azide 0.03%; 2,2-dimethyl-2-silapentane-5-sulfonic acid 0.2 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.9; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
1D 1H-15N HSQCsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

VNMR v6.1B, Varian - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - processing

NMR spectrometers:

  • Varian Unity 600 MHz

Related Database Links:

BMRB 16190 17103 19789 25034 25035 25120 25495
PDB
DBJ BAA02369 BAG36483 BAB92975 BAB92976 BAB92977 BAB92978 BAB92979
EMBL CAA30736 CAG46663 CAG46683 CAE45814 CAG46725
GB AAA36772 AAA37492 AAA37493 AAA48654 AAA49502 AAA36777 AAA96440 AAA96441 AAA96442 AAA96443
PIR S07450 TPHUCC TPRBTS
PRF 1403394A 1510257A 750650A 1810398A
REF NP_001029277 NP_001029523 NP_001123715 NP_001272501 NP_001291793 NP_001001441 NP_001001863 NP_001036245 NP_001036246 NP_001036247
SP P02591 P05936 P09860 P19123 P63315 P02641 P09739 P45378 Q75NG9 Q8MKI3
TPG DAA16908 DAA13525