BMRB Entry 16717

Title:
Backbone chemical shifts of NI3C DARPin
Deposition date:
2010-02-10
Original release date:
2010-08-12
Authors:
Wetzel, Svava; Ewald, Christina; Jurt, Simon; Zerbe, Oliver; Plueckthun, Andreas
Citation:

Citation: Wetzel, Svava; Ewald, Christina; Settanni, Giovanni; Jurt, Simon; Pluckthun, Andreas; Zerbe, Oliver. "Residue-Resolved Stability of Full-Consensus Ankyrin Repeat Proteins Probed by NMR."  J. Mol. Biol. 402, 241-258 (2010).
PubMed: 20654623

Assembly members:

Assembly members:
NI3C, polymer, 166 residues, 17896.91 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSW_NI3C

Data sets:
Data typeCount
13C chemical shifts346
15N chemical shifts149
1H chemical shifts149

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NI3C monomer1

Entities:

Entity 1, NI3C monomer 166 residues - 17896.91 Da.

1   METARGGLYSERHISHISHISHISHISHIS
2   GLYSERASPLEUGLYLYSLYSLEULEUGLU
3   ALAALAARGALAGLYGLNASPASPGLUVAL
4   ARGILELEUMETALAASNGLYALAASPVAL
5   ASNALALYSASPLYSASPGLYTYRTHRPRO
6   LEUHISLEUALAALAARGGLUGLYHISLEU
7   GLUILEVALGLUVALLEULEULYSALAGLY
8   ALAASPVALASNALALYSASPLYSASPGLY
9   TYRTHRPROLEUHISLEUALAALAARGGLU
10   GLYHISLEUGLUILEVALGLUVALLEULEU
11   LYSALAGLYALAASPVALASNALALYSASP
12   LYSASPGLYTYRTHRPROLEUHISLEUALA
13   ALAARGGLUGLYHISLEUGLUILEVALGLU
14   VALLEULEULYSALAGLYALAASPVALASN
15   ALAGLNASPLYSPHEGLYLYSTHRALAPHE
16   ASPILESERILEASPASNGLYASNGLUASP
17   LEUALAGLUILELEUGLN

Samples:

sample_1: NI3C, [U-99% 15N], 700 uM; potassium phosphate 50 mM; sodium chloride 150 mM; H2O 90%; D2O 10%

sample_2: NI3C, [U-100% 13C; U-100% 15N; U-80% 2H], 700 uM; potassium phosphate 50 mM; sodium chloride 150 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.2 M; pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CACBsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D HN(COCA)NHsample_2isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CARA v1.8.3, R. Keller - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks