BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16708

Title: 1H,13C,15N assignments of RNA recognition motifs 1 and 2 of BRUNOL-3   PubMed: 20443086

Authors: Bhatt, Harshesh; Kashyap, Maruthi; Bhavesh, Neel Sarovar

Citation: Bhatt, Harshesh; Kashyap, Maruthi; Bhavesh, Neel Sarovar. "1H, 13C and 15N NMR assignments of RNA recognizing motifs 1 and 2 of BRUNOL-3 protein from human involved in myotonic dystrophy."  Biomol. NMR Assignments 4, 143-145 (2010).

Assembly members:
BRUNOL-3, polymer, 199 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
BRUNOL-3: MNGALDHSDQPDPDAIKMFV GQIPRSWSEKELKELFEPYG AVYQINVLRDRSQNPPQSKG CCFVTFYTRKAALEAQNALH NIKTLPGMHHPIQMKPADSE KSNAVEDRKLFIGMVSKKCN ENDIRVMFSPFGQIEECRIL RGPDGLSRGCAFVTFSTRAM AQNAIKAMHQSQTMEGCSSP IVVKFADTQKDKEQRRLQQ

Data sets:
Data typeCount
13C chemical shifts798
15N chemical shifts197
1H chemical shifts1061

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BRUNOL-31

Entities:

Entity 1, BRUNOL-3 199 residues - Formula weight is not available

1   METASNGLYALALEUASPHISSERASPGLN
2   PROASPPROASPALAILELYSMETPHEVAL
3   GLYGLNILEPROARGSERTRPSERGLULYS
4   GLULEULYSGLULEUPHEGLUPROTYRGLY
5   ALAVALTYRGLNILEASNVALLEUARGASP
6   ARGSERGLNASNPROPROGLNSERLYSGLY
7   CYSCYSPHEVALTHRPHETYRTHRARGLYS
8   ALAALALEUGLUALAGLNASNALALEUHIS
9   ASNILELYSTHRLEUPROGLYMETHISHIS
10   PROILEGLNMETLYSPROALAASPSERGLU
11   LYSSERASNALAVALGLUASPARGLYSLEU
12   PHEILEGLYMETVALSERLYSLYSCYSASN
13   GLUASNASPILEARGVALMETPHESERPRO
14   PHEGLYGLNILEGLUGLUCYSARGILELEU
15   ARGGLYPROASPGLYLEUSERARGGLYCYS
16   ALAPHEVALTHRPHESERTHRARGALAMET
17   ALAGLNASNALAILELYSALAMETHISGLN
18   SERGLNTHRMETGLUGLYCYSSERSERPRO
19   ILEVALVALLYSPHEALAASPTHRGLNLYS
20   ASPLYSGLUGLNARGARGLEUGLNGLN

Samples:

sample_1: BRUNOL-3, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; sodium phosphate 20 ± 0.5 mM; sodium chloride 50 ± 0.5 mM; sodium azide 1 ± 0.05 %

sample_conditions_1: pH: 6.3; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNNsample_1isotropicsample_conditions_1
3D HN(C)Nsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2, Bruker Biospin - collection, processing

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 500 MHz

Related Database Links:

DBJ BAB87828 BAE23295 BAE31371 BAE36053 BAE37963
EMBL CAA09102 CAA09103 CAA77110 CAH91279 CAH91772
GB AAB09040 AAB09041 AAD02074 AAD13760 AAD13761
PIR JC7967
REF NP_001020247 NP_001020248 NP_001077055 NP_001077060 NP_001079593
SP A4IIM2 O95319 Q5R8Y8 Q6P0B1 Q792H5