BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16678

Title: 1H, 13C and 15N Chemical Shift Assignments for Sensory Rhodopsin II   PubMed: 20512150

Authors: Gautier, Antoine; Mott, Helen; Bostock, Mark; Kirkpatrick, John; Nietlispach, Daniel

Citation: Gautier, Antoine; Mott, Helen; Bostock, Mark; Kirkpatrick, John; Nietlispach, Daniel. "Structure determination of the seven-helix transmembrane receptor sensory rhodopsin II by solution NMR spectroscopy."  Nat. Struct. Mol. Biol. 17, 768-774 (2010).

Assembly members:
Sensory Rhodopsin II, polymer, 247 residues, 26723.572 Da.

Natural source:   Common Name: Natronomonas pharaonis   Taxonomy ID: 2257   Superkingdom: Archaea   Kingdom: not available   Genus/species: Natronomonas pharaonis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Sensory Rhodopsin II: MVGLTTLFWLGAIGMLVGTL AFAWAGRDAGSGERRYYVTL VGISGIAAVAYAVMALGVGW VPVAERTVFVPRYIDWILTT PLIVYFLGLLAGLDSREFGI VITLNTVVMLAGFAGAMVPG IERYALFGMGAVAFIGLVYY LVGPMTESASQRSSGIKSLY VRLRNLTVVLWAIYPFIWLL GPPGVALLTPTVDVALIVYL DLVTXVGFGFIALDAAATLR AEHGESLAGVDTDTPAVADL EHHHHHH

Data sets:
Data typeCount
13C chemical shifts1004
15N chemical shifts239
1H chemical shifts1530

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sensory Rhodopsin II1

Entities:

Entity 1, Sensory Rhodopsin II 247 residues - 26723.572 Da.

1   METVALGLYLEUTHRTHRLEUPHETRPLEU
2   GLYALAILEGLYMETLEUVALGLYTHRLEU
3   ALAPHEALATRPALAGLYARGASPALAGLY
4   SERGLYGLUARGARGTYRTYRVALTHRLEU
5   VALGLYILESERGLYILEALAALAVALALA
6   TYRALAVALMETALALEUGLYVALGLYTRP
7   VALPROVALALAGLUARGTHRVALPHEVAL
8   PROARGTYRILEASPTRPILELEUTHRTHR
9   PROLEUILEVALTYRPHELEUGLYLEULEU
10   ALAGLYLEUASPSERARGGLUPHEGLYILE
11   VALILETHRLEUASNTHRVALVALMETLEU
12   ALAGLYPHEALAGLYALAMETVALPROGLY
13   ILEGLUARGTYRALALEUPHEGLYMETGLY
14   ALAVALALAPHEILEGLYLEUVALTYRTYR
15   LEUVALGLYPROMETTHRGLUSERALASER
16   GLNARGSERSERGLYILELYSSERLEUTYR
17   VALARGLEUARGASNLEUTHRVALVALLEU
18   TRPALAILETYRPROPHEILETRPLEULEU
19   GLYPROPROGLYVALALALEULEUTHRPRO
20   THRVALASPVALALALEUILEVALTYRLEU
21   ASPLEUVALTHRLYRVALGLYPHEGLYPHE
22   ILEALALEUASPALAALAALATHRLEUARG
23   ALAGLUHISGLYGLUSERLEUALAGLYVAL
24   ASPTHRASPTHRPROALAVALALAASPLEU
25   GLUHISHISHISHISHISHIS

Samples:

sample_1: SENSORY RHODOPSIN II, [U-100% 15N], 0.5 mM; sodium chloride 50 mM; sodium phosphate 50 mM; sodium azide 0.05%

sample_2: SENSORY RHODOPSIN II, [U-100% 13C; U-100% 15N], 0.5 mM; sodium chloride 50 mM; sodium phosphate 50 mM; sodium azide 0.05%

sample_3: SENSORY RHODOPSIN II, [U-13C; U-15N; U-2H], 0.5 mM; sodium chloride 50 mM; sodium phosphate 50 mM; sodium azide 0.05%

sample_conditions_1: ionic strength: 100 mM; pH: 6; pressure: 1 atm; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HNCAsample_3isotropicsample_conditions_1
3D HN(CO)CAsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D HN(CA)COsample_3isotropicsample_conditions_1
3D HN(CA)CBsample_3isotropicsample_conditions_1
3D HN(COCA)CBsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

ARIA, Linge, O, . - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

ANALYSIS, CCPN - chemical shift assignment, chemical shift calculation, peak picking

AZARA, Boucher - processing

TALOS, Cornilescu, Delaglio and Bax - Torsion angle prediction

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz

Related Database Links:

PDB
EMBL CAA84469 CAI50508
REF WP_011324120
SP P42196