BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16635

Title: Solution structure of the N-terminal domain of Bacillus subtilis delta subunit of RNA polymerase   PubMed: 20310067

Authors: Motackova, Veronika; Sanderova, Hana; Zidek, Lukas; Novacek, Jiri; Padrta, Petr; Svenkova, Alzbeta; Jonak, Jiri; Krasny, Libor; Sklenar, Vladimir

Citation: Motackova, Veronika; Sanderova, Hana; Zidek, Lukas; Novacek, Jiri; Padrta, Petr; Svenkova, Alzbeta; Korelusova, Jana; Jonak, Jiri; Krasny, Libor; Sklenar, Vladimir. "Solution structure of the N-terminal domain of Bacillus subtilis delta subunit of RNA polymerase and its classification based on structural homologs."  Proteins 78, 1807-1810 (2010).

Assembly members:
N_delta, polymer, 99 residues, Formula weight is not available

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
N_delta: GIKQYSQEELKEMALVEIAH ELFEEHKKPVPFQELLNEIA SLLGVKKEELGDRIAQFYTD LNIDGRFLALSDQTWGLRSW YPYDQLDEETQLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts452
15N chemical shifts105
1H chemical shifts715
coupling constants87
residual dipolar couplings346

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N_delta1

Entities:

Entity 1, N_delta 99 residues - Formula weight is not available

1   GLYILELYSGLNTYRSERGLNGLUGLULEU
2   LYSGLUMETALALEUVALGLUILEALAHIS
3   GLULEUPHEGLUGLUHISLYSLYSPROVAL
4   PROPHEGLNGLULEULEUASNGLUILEALA
5   SERLEULEUGLYVALLYSLYSGLUGLULEU
6   GLYASPARGILEALAGLNPHETYRTHRASP
7   LEUASNILEASPGLYARGPHELEUALALEU
8   SERASPGLNTHRTRPGLYLEUARGSERTRP
9   TYRPROTYRASPGLNLEUASPGLUGLUTHR
10   GLNLEUGLUHISHISHISHISHISHIS

Samples:

sample_1: N_delta mM; sodium chloride 10 mM; sodium azide 50 uM; phosphate buffer 20 mM

sample_2: N_delta mM; sodium chloride 10 mM; sodium azide 50 uM; phosphate buffer 20 mM; filamentous Pf1 bacteriophage 14.29 mg/ml

sample_3: N_delta mM; sodium chloride 10 mM; sodium azide 50 uM; phosphate buffer 20 mM; polyacrylamide gel 5%

sample_conditions_1: ionic strength: 10 mM; pH: 6.6; pressure: 1 atm; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HMQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
(HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
2D 1H-15N IPAPsample_2anisotropicsample_conditions_1
2D HN[C]-S3Esample_2anisotropicsample_conditions_1
2D (H)CACO-IPAPsample_2anisotropicsample_conditions_1
2D 13C-coupled 1H-13C HSQCsample_2anisotropicsample_conditions_1
2D 1H-15N IPAPsample_3anisotropicsample_conditions_1
2D HN[C]-S3Esample_3anisotropicsample_conditions_1
2D (H)CACO-IPAPsample_3anisotropicsample_conditions_1
2D 13C-coupled 1H-13C HSQCsample_3anisotropicsample_conditions_1
2D 13C-detected CONsample_1isotropicsample_conditions_1
2D 13C-detected CONsample_2anisotropicsample_conditions_1
2D 1H-15N IPAPsample_1isotropicsample_conditions_1
2D HN[C]-S3Esample_1isotropicsample_conditions_1
2D (H)CACO-IPAPsample_1isotropicsample_conditions_1
2D 13C-coupled 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY v3.111, Goddard - chemical shift assignment, peak picking

ARIA v2.1, Linge, O, . - NOE assignment

CNS vTENSO, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

X-PLOR, Brunger - refinement

S3EPY, Petr Novak - dipolar coupling determination, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 16912 18903 19284
PDB
DBJ BAM55799 BAM59811 BAO93636 GAK81030
EMBL CAA89869 CAB15744 CCU60814 CEI59524 CEJ79381
GB AAA22710 ADM39706 ADV94526 AEP88664 AEP92774
REF NP_391597 WP_003221959 WP_003227609 WP_003235832 WP_003243661
SP P12464