BMRB Entry 16614

Title:
1H, 13C and 15N resonance assignments of a highly-soluble murine interleukin-3 analogue with wild-type bioactivity
Deposition date:
2009-11-22
Original release date:
2010-05-05
Authors:
Yao, Shenggen; Murphy, James; Low, Andrew; Norton, Raymond
Citation:

Citation: Yao, Shenggen; Murphy, James; Low, Andrew; Norton, Raymond. "1H, 13C and 15N resonance assignments of a highly-soluble murine interleukin-3 analogue with wild-type bioactivity."  Biomol. NMR Assignments 4, 73-77 (2010).
PubMed: 20174897

Assembly members:

Assembly members:
mIL3, polymer, 127 residues, 14286 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETM60

Data sets:
Data typeCount
13C chemical shifts460
15N chemical shifts125
1H chemical shifts793

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mIL3(33-156)1

Entities:

Entity 1, mIL3(33-156) 127 residues - 14286 Da.

The first 3 residues are cloning artifacts

1   GLYALAMETASPTHRHISARGLEUTHRARG
2   THRLEUASNCYSSERSERILEVALLYSGLU
3   ILEILEGLYLYSLEUPROGLUPROGLULEU
4   LYSTHRASPASPGLUGLYPROSERLEUARG
5   ASNLYSSERPHEARGARGVALASNLEUSER
6   LYSPHEVALGLUSERGLNGLYGLUVALASP
7   PROGLUASPARGTYRVALILELYSSERASN
8   LEUGLNLYSLEUASNALACYSLEUPROTHR
9   SERALAASNASPSERALALEUPROGLYVAL
10   PHEILEARGASPLEUASPASPPHEARGLYS
11   LYSLEUARGPHETYRMETVALHISLEUASN
12   ASPLEUGLUTHRVALLEUTHRSERARGPRO
13   PROGLNPROALASERGLYSER

Samples:

sample_1: mIL3, [U-100% 15N], 0.28 mM; potassium phosphate 20 mM; sodium azide 0.2%; H2O 95%; D2O 5%

sample_2: mIL3, [U-100% 13C; U-100% 15N], 0.28 mM; potassium phosphate 20 mM; sodium azide 0.2%; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.02 M; pH: 6.7; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

TOPSPIN v1.3, Bruker Biospin - processing

XEASY v1.3, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAE32129
EMBL CAA26514
GB AAA39291 AAA39293 AAA39308 AAA39507 AAB24323
PRF 1002253A
REF NP_034686
SP P01586
AlphaFold P01586

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks