BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16601

Title: kkgf   PubMed: 22593157

Authors: Montserret, Roland; Penin, Francois

Citation: Oehler, Verena; Filipe, Ana; Montserret, Roland; da Costa, Daniel; Brown, Gaie; Penin, Francois; McLauchlan, John. "Structural analysis of hepatitis C virus core-e1 signal Peptide and requirements for cleavage of the genotype 3a signal sequence by signal Peptide peptidase."  J. Virol. 86, 7818-7828 (2012).

Assembly members:
HCV_Core-E1, polymer, 27 residues, 2881.567 Da.

Natural source:   Common Name: Hepatitis C virus   Taxonomy ID: 11103   Superkingdom: virus   Kingdom: not available   Genus/species: not available Hepatitis C

Experimental source:   Production method: chemical synthesis   Host organism: none

Entity Sequences (FASTA):
HCV_Core-E1: KKGFPFSIFLLALLSCITVP VSAAQVK

Data sets:
Data typeCount
13C chemical shifts75
1H chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HCV_Core-E11

Entities:

Entity 1, HCV_Core-E1 27 residues - 2881.567 Da.

1   LYSLYSGLYPHEPROPHESERILEPHELEU
2   LEUALALEULEUSERCYSILETHRVALPRO
3   VALSERALAALAGLNVALLYS

Samples:

sample_1: kkgf 2 ± 0.1 mM; DSS 0.01 mM; H2O 50 %v/v; trifluoroethanol d2OH 50 %v/v

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - data collection

SPARKY, Goddard - chemical shift assignment

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

Molmol, Koradi, Billeter and Wuthrich - structures superposition

TALOS, Cornilescu, Delaglio and Bax - dihedral angle calculations

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

BMRB 17891
PDB
DBJ BAB32872 BAF34893 BAF95986
GB ABN54499 ABN54513 ABN54545 ABN54546 ABN54549
SP Q99IB8