BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16596

Title: First PBZ domain of human APLF protein   PubMed: 20098424

Authors: Neuhaus, David; Eustermann, Sebastian; Brockmann, Christoph; Yang, Ji-Chun

Citation: Eustermann, Sebastian; Brockmann, Christoph; Mehrotra, Pawan Vinod; Yang, Ji-Chun; Loakes, David; West, Stephen; Ahel, Ivan; Neuhaus, David. "Solution structures of the two PBZ domains from human APLF and their interaction with poly(ADP-ribose)."  Nat. Struct. Mol. Biol. 17, 241-243 (2010).

Assembly members:
APLF_363-451, polymer, 89 residues, 5952.459 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
APLF_363-451: GPLGSGSEGNKVKRTSCMYG ANCYRKNPVHFQHFSHPGDS DYGGVQIVGQDETDDRPECP YGPSCYRKNPQHKIEYRHNT LPVRNVLDE

Data sets:
Data typeCount
13C chemical shifts288
15N chemical shifts87
1H chemical shifts562

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1APLF_363-4511
2ZINC ION, 12
3ZINC ION, 22

Entities:

Entity 1, APLF_363-451 89 residues - 5952.459 Da.

1   GLYPROLEUGLYSERGLYSERGLUGLYASN
2   LYSVALLYSARGTHRSERCYSMETTYRGLY
3   ALAASNCYSTYRARGLYSASNPROVALHIS
4   PHEGLNHISPHESERHISPROGLYASPSER
5   ASPTYRGLYGLYVALGLNILEVALGLYGLN
6   ASPGLUTHRASPASPARGPROGLUCYSPRO
7   TYRGLYPROSERCYSTYRARGLYSASNPRO
8   GLNHISLYSILEGLUTYRARGHISASNTHR
9   LEUPROVALARGASNVALLEUASPGLU

Entity 2, ZINC ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: potassium pyrophosphate 20 mM; sodium chloride 200 mM; zinc sulphate 100 uM; DTT, [U-2H], 2 mM; APLF_363-451, [U-98% 13C; U-98% 15N], 0.5-0.6 mM

sample_2: potassium pyrophosphate 20 mM; sodium chloride 200 mM; zinc sulphate 100 uM; DTT, [U-2H], 2 mM; APLF_363-451, [U-98% 13C; U-98% 15N], 0.5-0.6 mM

sample_conditions_1: ionic strength: 0.4 M; pH: 6.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HMQC long-rangesample_1isotropicsample_conditions_1
2D 1H-13C HSQC full-widthsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESY filteredsample_1isotropicsample_conditions_1
3D CBCANHsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHANHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(C)CH-TOCSYsample_1isotropicsample_conditions_1
3D (H)CCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1
3D HACAHB-COSYsample_2isotropicsample_conditions_1

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

SPARKY, Goddard - chemical shift assignment

CCPN_analysis, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker DMX 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

BMRB 16744
PDB
DBJ BAF83530
GB AAH41144 AAY24113 ADZ15607 AIC53375 EAW99871
REF NP_775816 XP_002799328 XP_003830966 XP_004029419 XP_005575758
SP Q8IW19