BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16566

Title: D-G3P-bound WT yeast Triosephosphate Isomerase (TIM)   PubMed: 19854202

Authors: Xu, Yimin; Lorieau, Justin; McDermott, Ann

Citation: Xu, Yimin; Lorieau, Justin; McDermott, Ann. "Triosephosphate Isomerase: (15)N and (13)C Chemical Shift Assignments and Conformational Change upon Ligand Binding by Magic-Angle Spinning Solid-State NMR Spectroscopy."  J. Mol. Biol. 397, 233-248 (2010).

Assembly members:
WT_yeast_TIM_homodimer, polymer, 248 residues, Formula weight is not available
G3P, non-polymer, 172.074 Da.

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
WT_yeast_TIM_homodimer: MARTFFVGGNFKLNGSKQSI KEIVERLNTASIPENVEVVI CPPATYLDYSVSLVKKPQVT VGAQNAYLKASGAFTGENSV DQIKDVGAKWVILGHSERRS YFHEDDKFIADKTKFALGQG VGVILCIGETLEEKKAGKTL DVVERQLNAVLEEVKDWTNV VVAYEPVWAIGTGLAATPED AQDIHASIRKFLASKLGDKA ASELRILYGGSANGSNAVTF KDKADVDGFLVGGASLKPEF VDIINSRN

Data sets:
Data typeCount
13C chemical shifts296
15N chemical shifts20

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1WT TIM chain 11
2WT TIM chain 21
3G3P, 12
4G3P, 22

Entities:

Entity 1, WT TIM chain 1 248 residues - Formula weight is not available

1   METALAARGTHRPHEPHEVALGLYGLYASN
2   PHELYSLEUASNGLYSERLYSGLNSERILE
3   LYSGLUILEVALGLUARGLEUASNTHRALA
4   SERILEPROGLUASNVALGLUVALVALILE
5   CYSPROPROALATHRTYRLEUASPTYRSER
6   VALSERLEUVALLYSLYSPROGLNVALTHR
7   VALGLYALAGLNASNALATYRLEULYSALA
8   SERGLYALAPHETHRGLYGLUASNSERVAL
9   ASPGLNILELYSASPVALGLYALALYSTRP
10   VALILELEUGLYHISSERGLUARGARGSER
11   TYRPHEHISGLUASPASPLYSPHEILEALA
12   ASPLYSTHRLYSPHEALALEUGLYGLNGLY
13   VALGLYVALILELEUCYSILEGLYGLUTHR
14   LEUGLUGLULYSLYSALAGLYLYSTHRLEU
15   ASPVALVALGLUARGGLNLEUASNALAVAL
16   LEUGLUGLUVALLYSASPTRPTHRASNVAL
17   VALVALALATYRGLUPROVALTRPALAILE
18   GLYTHRGLYLEUALAALATHRPROGLUASP
19   ALAGLNASPILEHISALASERILEARGLYS
20   PHELEUALASERLYSLEUGLYASPLYSALA
21   ALASERGLULEUARGILELEUTYRGLYGLY
22   SERALAASNGLYSERASNALAVALTHRPHE
23   LYSASPLYSALAASPVALASPGLYPHELEU
24   VALGLYGLYALASERLEULYSPROGLUPHE
25   VALASPILEILEASNSERARGASN

Entity 2, G3P, 1 - C3 H9 O6 P - 172.074 Da.

1   G3P

Samples:

sample_1: WT yeast TIM homodimer, [U-100% 13C; U-100% 15N], 7 mM; D-glycerol 3-phosphate 35 mM; Tris-HCl 50 mM; NaCl 50 mM; EDTA 1 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DARRsample_1solidsample_conditions_1
2D 15N-13C DCPsample_1solidsample_conditions_1
3D 15N-13C-13C DCP-DARRsample_1solidsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

NMR spectrometers:

  • Bruker DRX 750 MHz
  • Bruker DRX 900 MHz

Related Database Links:

BMRB 16565 17122
PDB
DBJ GAA22287
EMBL CAA89080 CAY78558
GB AAA88757 AAS55980 AHY75046 AJP37778 AJU57896
REF NP_010335
SP P00942
TPG DAA11897