BMRB Entry 16550

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PDB ID: 2kpa
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16550
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO

Deposition date:

2009-10-11

Original release date:

2010-02-18

Authors:

Merkulova, Maria; Bakulina, Anastasia; Thaker, Youg; Gruber, Gerhard; Marshansky, Vladimir

Citation:

Citation: Merkulova, Maria; Bakulina, Anastasia; Thaker, Youg Raj; Gruber, Gerhard; Marshansky, Vladimir. "Specific motifs of the V-ATPase a2-subunit isoform interact with catalytic and regulatory domains of ARNO." Biochim. Biophys. Acta 1797, 1398-1409 (2010).
PubMed: 20153292

Assembly members:

Assembly members:
ARNO(375-400), polymer, 26 residues, 3076 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ARNO(375-400): VSVDPFYEMLAARKKRISVK KKQEQP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	156

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PB domain	1

Entities:

Entity 1, PB domain 26 residues - 3076 Da.

Residues from 375-400 represents PB (Polybasic)of ARNO protein.

1

VAL

SER

VAL

ASP

PRO

PHE

TYR

GLU

MET

LEU

2

ALA

ARG

LYS

ARG

ILE

SER

VAL

LYS

3

LYS

GLN

GLU

GLN

PRO

Samples:

sample_1: ARNO(375-400), [U-100% 15N], 1-2 mM

sample_conditions_1: pH: 7.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1

Software:

SPARKY v2.6.9, Goddard - chemical shift assignment, chemical shift calculation, data analysis

NMR spectrometers:

Bruker Avance 600 MHz

PDB	2KPA 2KPB
DBJ	BAA33429 BAA33430 BAA33431 BAA33432 BAA87927
EMBL	CAA68084
GB	AAB09591 AAB41444 AAC77924 AAH04361 AAH04662
REF	NP_001070562 NP_001106171 NP_001135984 NP_001137592 NP_001247820
SP	P63034 P63035 Q2KI41 Q76MY7 Q99418
TPG	DAA19658
AlphaFold	Q76MY7 P63034 P63035 Q2KI41 Q99418