BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16534

Title: DICER LIKE protein   PubMed: 20106953

Authors: Qin, Haina; Song, Jianxing; Yuan, Adam, Yu-Ren

Citation: Qin, Haina; Chen, Fading; Huan, Xuelu; Machida, Satoru; Song, Jianxing; Yuan, Y. Adam. "Structure of the Arabidopsis thaliana DCL4 DUF283 domain reveals a noncanonical double-stranded RNA-binding fold for protein-protein interaction."  RNA 16, 474-481 (2010).

Assembly members:
DCL4_DUF283, polymer, 102 residues, Formula weight is not available

Natural source:   Common Name: thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DCL4_DUF283: ISGGSSISMMYKYCSRLPHD EFFQPKPEFQFKPVDEFGGT ICRITLPANAPISEIESSLL PSTEAAKKDACLKAVHELHN LGVLNDFLLPDSKDEIEDEL SD

Data sets:
Data typeCount
13C chemical shifts246
15N chemical shifts85
1H chemical shifts514

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DCL4_DUF2831

Entities:

Entity 1, DCL4_DUF283 102 residues - Formula weight is not available

1   ILESERGLYGLYSERSERILESERMETMET
2   TYRLYSTYRCYSSERARGLEUPROHISASP
3   GLUPHEPHEGLNPROLYSPROGLUPHEGLN
4   PHELYSPROVALASPGLUPHEGLYGLYTHR
5   ILECYSARGILETHRLEUPROALAASNALA
6   PROILESERGLUILEGLUSERSERLEULEU
7   PROSERTHRGLUALAALALYSLYSASPALA
8   CYSLEULYSALAVALHISGLULEUHISASN
9   LEUGLYVALLEUASNASPPHELEULEUPRO
10   ASPSERLYSASPGLUILEGLUASPGLULEU
11   SERASP

Samples:

sample_1: protein, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.02 M; pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - refinement

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAZ80387 AED92830 AED92831
REF NP_001190348 NP_197532
SP P84634