BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16509

Title: Complement Repeats 16 17 and 18 from LRP-1   PubMed: 20030366

Authors: Guttman, Miklos; Komives, Elizabeth

Citation: Guttman, Miklos; Prieto, J. Helena; Croy, Johnny; Komives, Elizabeth. "Decoding of lipoprotein-receptor interactions: properties of ligand binding modules governing interactions with apolipoprotein E."  Biochemistry 49, 1207-1216 (2010).

Assembly members:
CR1618, polymer, 129 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CR1618: GSNKFCSEAQFECQNHRCIS KQWLCDGSDDCGDGSDEAAH CEGKTCGPSSFSCPGTHVCV PERWLCDGDKDCADGADESI AAGCLYNSTCDDREFMCQNR QCIPKHFVCDHDRDCADGSD ESPECEYPT

Data sets:
Data typeCount
13C chemical shifts193
15N chemical shifts101
1H chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CR16181
2Calcium ion2

Entities:

Entity 1, CR1618 129 residues - Formula weight is not available

There's an extra GS at the N terminus, from the expression vector.

1   GLYSERASNLYSPHECYSSERGLUALAGLN
2   PHEGLUCYSGLNASNHISARGCYSILESER
3   LYSGLNTRPLEUCYSASPGLYSERASPASP
4   CYSGLYASPGLYSERASPGLUALAALAHIS
5   CYSGLUGLYLYSTHRCYSGLYPROSERSER
6   PHESERCYSPROGLYTHRHISVALCYSVAL
7   PROGLUARGTRPLEUCYSASPGLYASPLYS
8   ASPCYSALAASPGLYALAASPGLUSERILE
9   ALAALAGLYCYSLEUTYRASNSERTHRCYS
10   ASPASPARGGLUPHEMETCYSGLNASNARG
11   GLNCYSILEPROLYSHISPHEVALCYSASP
12   HISASPARGASPCYSALAASPGLYSERASP
13   GLUSERPROGLUCYSGLUTYRPROTHR

Entity 2, Calcium ion - Ca - 40.078 Da.

1   CA

Samples:

sample_1: entity, [U-99% 13C; U-99% 15N], 0.7 mM; sodium chloride 150 mM; sodium azide 3 mM; CA 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.15 M; pH: 6.6; pressure: 1 atm; temperature: 307 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

AZARA v2.7, Boucher - processing

FELIX v2000, Accelrys Software Inc. - chemical shift assignment, peak picking, processing

SPARKY v3.113, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 800 MHz

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