BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16365

Title: Solution NMR structure of yeast protein YOR252W [residues 38-178]: Northeast Structural Genomics Consortium target YT654

Authors: Cort, John; Yee, Adelinda; Liu, Chengsong; Arrowsmith, Cheryl; Kennedy, Michael

Citation: Cort, John; Yee, Adelinda; Liu, Chengsong; Arrowsmith, Cheryl; Kennedy, Michael. "Solution NMR structure of yeast protein YOR252W"  Not known ., .-..

Assembly members:
YOR252W, polymer, 141 residues, Formula weight is not available

Natural source:   Common Name: baker   Taxonomy ID: 4932   Superkingdom: not available   Kingdom: not available   Genus/species: Eukaryota Fungi

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
YOR252W: MREDKIAAKKKLHQDKRVHE LARVKFMQDVVNSDTFKGQP IFDHAHTREFIQSFIERDDT ELDELKKKRRSNRPPSNRQV LLQQRRDQELKEFKAGFLCP DLSDAKNMEFLRNWNGTFGL LNTLRLIRINDKGEQVVGGN E

Data sets:
Data typeCount
13C chemical shifts514
15N chemical shifts133
1H chemical shifts775

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YOR252W1

Entities:

Entity 1, YOR252W 141 residues - Formula weight is not available

An N-term His purification tag was cleaved.

1   METARGGLUASPLYSILEALAALALYSLYS
2   LYSLEUHISGLNASPLYSARGVALHISGLU
3   LEUALAARGVALLYSPHEMETGLNASPVAL
4   VALASNSERASPTHRPHELYSGLYGLNPRO
5   ILEPHEASPHISALAHISTHRARGGLUPHE
6   ILEGLNSERPHEILEGLUARGASPASPTHR
7   GLULEUASPGLULEULYSLYSLYSARGARG
8   SERASNARGPROPROSERASNARGGLNVAL
9   LEULEUGLNGLNARGARGASPGLNGLULEU
10   LYSGLUPHELYSALAGLYPHELEUCYSPRO
11   ASPLEUSERASPALALYSASNMETGLUPHE
12   LEUARGASNTRPASNGLYTHRPHEGLYLEU
13   LEUASNTHRLEUARGLEUILEARGILEASN
14   ASPLYSGLYGLUGLNVALVALGLYGLYASN
15   GLU

Samples:

sample_1: YOR252W, [U-99% 13C; U-99% 15N], 0.6 mM; sodium chloride 300 mM; DTT 10 mM; benzamidine 1 mM; sodium azide 0.01%; H2O 93%; D2O 7%

sample_2: YOR252W, [U-99% 13C; U-99% 15N], 0.6 mM; sodium chloride 300 mM; DTT 10 mM; benzamidine 1 mM; sodium azide 0.01%; D2O 100%

sample_conditions_1: ionic strength: 300 mM; pH: 7; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
4D 1H-13C NOESYsample_2isotropicsample_conditions_1

Software:

AutoStruct, Huang, Tejero, Powers and Montelione - refinement, structure solution

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, refinement

PSVS, Bhattacharya and Montelione - refinement

SPARKY, Goddard - chemical shift assignment, peak picking

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ GAA26563
EMBL CAA99474 CAY86533
GB AHY77532 AJP41762 AJT71184 AJT71672 AJT72162
REF NP_014895
SP Q08687
TPG DAA11019