BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16332

Title: pH dependent structures of LAH4 in micellar environmnet:mode of acting

Authors: Georgescu, Julia; Bechinger, Burkhard

Citation: Georgescu, Julia; Bechinger, Burkhard. "Structure-function relationships of the polyhistidine-rich peptide LAH4 in micellar environments; pH dependent mode of antibiotic action & DNA transfection"  Biophys. J. ., .-..

Assembly members:
lah4, polymer, 26 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
lah4: KKALLALALHHLAHLALHLA LALKKA

Data sets:
Data typeCount
1H chemical shifts174

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer1

Entities:

Entity 1, monomer 26 residues - Formula weight is not available

1   LYSLYSALALEULEUALALEUALALEUHIS
2   HISLEUALAHISLEUALALEUHISLEUALA
3   LEUALALEULYSLYSALA

Samples:

sample_1: lah4 2 mM; H2O 90%; D2O 10%; sodium chloride 115 mM; sodium phosphate 20 mM; potassium dihydrogen phosphate 8 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 4.1; pressure: 1 atm; temperature: 317 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D ROESYsample_1isotropicsample_conditions_1

Software:

ccnmr, Bruker Biospin, Brunger, Brunger, Adams, Clore, Gros, Nilges and Read, Cieslar C. et al, Koradi, Billeter and Wuthrich, Laskowski and MacArthur, Rullmann, Doreleijers and Kaptein - chemical shift assignment

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 16333
PDB