BMRB Entry 16321

Title:
Backbone 1H, 13C and 15N resonance assignments for the E2 conjugating enzyme, UbcH8
Deposition date:
2009-05-28
Original release date:
2009-10-14
Authors:
Serniwka, Stephanie; Shaw, Gary
Citation:

Citation: Serniwka, Stephanie; Shaw, Gary. "The structure of the UbcH8-ubiquitin complex shows a unique ubiquitin interaction site"  Biochemistry 48, 12169-12179 (2009).
PubMed: 19928833

Assembly members:

Assembly members:
UbcH8, polymer, 152 residues, 17638 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: p11

Data sets:
Data typeCount
13C chemical shifts460
15N chemical shifts140
1H chemical shifts446

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UbcH81

Entities:

Entity 1, UbcH8 152 residues - 17638 Da.

1   METALASERMETARGVALVALLYSGLULEU
2   GLUASPLEUGLNLYSLYSPROPROPROTYR
3   LEUARGASNLEUSERSERASPASPALAASN
4   VALLEUVALTRPHISALALEULEULEUPRO
5   ASPGLNPROPROTYRHISLEULYSALAPHE
6   ASNLEUARGILESERPHEPROPROGLUTYR
7   PROPHELYSPROPROMETILELYSPHETHR
8   THRLYSILETYRHISPROASNVALASPGLU
9   ASNGLYGLNILECYSLEUPROILEILESER
10   SERGLUASNTRPLYSPROSERTHRLYSTHR
11   SERGLNVALLEUGLUALALEUASNVALLEU
12   VALASNARGPROASNILEARGGLUPROLEU
13   ARGMETASPLEUALAASPLEULEUTHRGLN
14   ASNPROGLULEUPHEARGLYSASNALAGLU
15   GLUPHETHRLEUARGPHEGLYVALASPARG
16   PROSER

Samples:

sample_UbcH8: UbcH8, [U-100% 13C; U-100% 15N], 0.5 mM; sodium phosphate 20 mM; EDTA 1 mM; DTT 1 mM; sodium chloride 150 mM; Arginine 50 mM; Glutamic Acid 50 mM; H2O 90%; D2O 10%

sample_conditions_for_UbcH8: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_UbcH8isotropicsample_conditions_for_UbcH8
3D CBCA(CO)NHsample_UbcH8isotropicsample_conditions_for_UbcH8
3D HNCACBsample_UbcH8isotropicsample_conditions_for_UbcH8
3D HNCOsample_UbcH8isotropicsample_conditions_for_UbcH8
3D C(CO)NHsample_UbcH8isotropicsample_conditions_for_UbcH8
3D H(CCO)NHsample_UbcH8isotropicsample_conditions_for_UbcH8

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ v2.1B, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

SWS O14933
PDB
DBJ BAG38109
EMBL CAB64566 CAG33407
GB AAB86433 AAD17525 AAH32491 ABM82217 ABM85401
REF NP_004214 NP_937826 XP_003318029 XP_003826475 XP_004051215
SP O14933
AlphaFold O14933

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks