BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16291

Title: d(AGAGCTCT)2 plus Ru ligand 1:2 assignments   PubMed: 20108276

Authors: Waywell, Phil; Thomas, James

Citation: Waywell, Philip; Gonzalez, Veronica; Gill, Martin; Adams, Harry; Meijer, Anthony J H M; Williamson, Mike; Thomas, James. "Structure of the Complex of [Ru(tpm)(dppz)py](2+) with a B-DNA Oligonucleotide-A Single-Substituent Binding Switch for a Metallo-Intercalator."  Chemistry 16, 2407-2417 (2010).

Assembly members:
d(AGAGCTCT)2, polymer, 8 residues, Formula weight is not available
Ru(tpm)(dppz)py, non-polymer, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
d(AGAGCTCT)2: AGAGCTCT

Data sets:
Data typeCount
1H chemical shifts55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1d(AGAGCTCT),11
2d(AGAGCTCT),21
3Ru(tpm)(dppz)py,12
4Ru(tpm)(dppz)py,22

Entities:

Entity 1, d(AGAGCTCT),1 8 residues - Formula weight is not available

1   DADGDADGDCDTDCDT

Entity 2, Ru(tpm)(dppz)py,1 - . - Formula weight is not available

1   RuTDpy

Samples:

sample_1: d(AGAGCTCT)2 2 mM; Ru(tpm)(dppz)py 4 mM; D2O 100%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 31P-1H COSYsample_1isotropicsample_conditions_1

Software:

FELIX, Accelrys Software Inc. - chemical shift assignment, processing

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 800 MHz

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