BMRB Entry 16217

Title:
Backbone 1H, 13CA, and 15N Chemical Shifts Assignments and 15N T1 and T2 Relaxation Parameters for the N-terminal (1-119) Fragment of sperm whale apomyoglobin
Deposition date:
2009-03-16
Original release date:
2012-08-06
Authors:
Rajagopalan, Senapathy; Cavagnero, Silvia; Kurt, Nese; Kwon, Jay
Citation:

Citation: Rajagopalan, Senapathy; Cavagnero, Silvia; Kurt, Nese; Kwon, Jay. "Backbone Dynamics of a Large Chaperone-Free and Chaperone-Bound Hsp70 Substrate"  Arch. Biochem. Biophys. ., .-..

Assembly members:

Assembly members:
apomyoglobin, polymer, 119 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET

Data sets:
Data typeCount
13C chemical shifts38
15N chemical shifts35
1H chemical shifts35
T1 relaxation values20
T2 relaxation values19

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apoMb(1-119) fragment in the presence of DnaK-beta1

Entities:

Entity 1, apoMb(1-119) fragment in the presence of DnaK-beta 119 residues - Formula weight is not available

1   VALLEUSERGLUGLYGLUTRPGLNLEUVAL
2   LEUHISVALTRPALALYSVALGLUALAASP
3   VALALAGLYHISGLYGLNASPILELEUILE
4   ARGLEUPHELYSSERHISPROGLUTHRLEU
5   GLULYSPHEASPARGPHELYSHISLEULYS
6   THRGLUALAGLUMETLYSALASERGLUASP
7   LEULYSLYSHISGLYVALTHRVALLEUTHR
8   ALALEUGLYALAILELEULYSLYSLYSGLY
9   HISHISGLUALAGLULEULYSPROLEUALA
10   GLNSERHISALATHRLYSHISLYSILEPRO
11   ILELYSTYRLEUGLUPHEILESERGLUALA
12   ILEILEHISVALLEUHISSERARGHIS

Samples:

sample_1: ApoMb(1-119) mM; Sodium Acetate 20 mM; potassium chloride 5 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 5 mM; pH: 6.0; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

NMRPIPE_and_NMRVIEW, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 1027 1029 1200 1413 1455 1457 1459 1461 1463 1465 1467 1469 1471 15589 16218 16499 16500 16501 1752 2345 2346 2347 2348 2431 2432 2433 2434 291 292 293 40 4061 4062 426 4568 4676 4695
PDB
DBJ BAF03579 BAF03582
GB AAA72199
PRF 742482A
REF NP_001277651
SP P02184 P02185 Q0KIY5
AlphaFold P02184 P02185 Q0KIY5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks