BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16178

Title: MeVamp-4   PubMed: 20097251

Authors: Du, Weihong; Xu, Jia; Gao, Bing; Zhu, Shunyi

Citation: Gao, Bing; Xu, Jia; Rodriguezc, M. del; Lanz-Mendozac, Humberto; Hernandez-Rivas, Rosaura; Du, Weihong; Zhu, Shunyi. "Characterization of two linear cationic antimalarial peptides in the scorpion Mesobuthus eupeus"  Biochimie 92, 350-359 (2010).

Assembly members:
MeVamp-4, polymer, 24 residues, 2761.265 Da.

Natural source:   Common Name: lesser Asian scorpion   Taxonomy ID: 34648   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mesobuthus eupeus

Experimental source:   Production method: chemical synthesis   Host organism: Mesobuthus eupeus

Entity Sequences (FASTA):
MeVamp-4: GRGREFMSNLKEKLSGVKEK MKNS

Data sets:
Data typeCount
1H chemical shifts170

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MeVamp-41

Entities:

Entity 1, MeVamp-4 24 residues - 2761.265 Da.

1   GLYARGGLYARGGLUPHEMETSERASNLEU
2   LYSGLULYSLEUSERGLYVALLYSGLULYS
3   METLYSASNSER

Samples:

sample_1: MeVamp-4 1 mM; d3-trifluoroethanol 50%; H2O 50%

sample_conditions_1: pH: 2.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

SPARKY v3.114, Goddard - chemical shift assignment, peak picking

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
GB ABR20112 ABR21034 ABR21074 AIL48788
SP P0CH57