BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16111

Title: Sequence-specific 1H, 15N and 13C resonance assignments of Art v 1: a proline-rich allergen of Artemisia vulgaris pollen   PubMed: 19636957

Authors: Razzera, Guilherme; Gadermaier, Gabriele; Almeida, Marcius; Ferreira, Fatima; Almeida, Fabio; Valente, Ana Paula

Citation: Razzera, Guilherme; Gadermaier, Gabriele; Almeida, Marcius; Ferreira, Fatima; Almeida, Fabio; Valente, Ana Paula. "Sequence-specific 1H, 15N and 13C resonance assignments of Art v 1: a proline-rich allergen of Artemisia vulgaris pollen"  Biomol. NMR Assignments 3, 103-106 (2009).

Assembly members:
Art_v_1, polymer, 108 residues, 10800.0 Da.

Natural source:   Common Name: common wormwood   Taxonomy ID: 4220   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Artemisia vulgaris

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Art_v_1: AGSKLCEKTSKTYSGKCDNK KCDKKCIEWEKAQHGACHKR EAGKESCFCYFDCSKSPPGA TPAPPGAAPPPAAGGSPSPP ADGGSPPPPADGGSPPVDGG SPPPPSTH

Data sets:
Data typeCount
13C chemical shifts293
15N chemical shifts106
1H chemical shifts603

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Art v 11

Entities:

Entity 1, Art v 1 108 residues - 10800.0 Da.

Proline-rich allergen of Artemisia vulgaris pollen

1   ALAGLYSERLYSLEUCYSGLULYSTHRSER
2   LYSTHRTYRSERGLYLYSCYSASPASNLYS
3   LYSCYSASPLYSLYSCYSILEGLUTRPGLU
4   LYSALAGLNHISGLYALACYSHISLYSARG
5   GLUALAGLYLYSGLUSERCYSPHECYSTYR
6   PHEASPCYSSERLYSSERPROPROGLYALA
7   THRPROALAPROPROGLYALAALAPROPRO
8   PROALAALAGLYGLYSERPROSERPROPRO
9   ALAASPGLYGLYSERPROPROPROPROALA
10   ASPGLYGLYSERPROPROVALASPGLYGLY
11   SERPROPROPROPROSERTHRHIS

Samples:

sample_1: Art v 1, [U-99% 13C; U-99% 15N], 1 mM; Potassium Acetate 10 mM

sample_conditions_1: ionic strength: 0.01 M; pH: 5.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
HCANsample_1isotropicsample_conditions_1
HCACONsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA v1.84, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
GB AAO24900 AAQ07455 AHF71025
SP Q84ZX5