BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16091

Title: SOLUTION STRUCTURE OF 30S RIBOSOMAL PROTEIN S8E; FROM Methanothermobacter thermautotrophicus, NORTHEASTSTRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET TR71D

Authors: Liu, Gaohua; Rossi, Paolo; Wang, Dongyan; NWOSU, CHIOMA; OWENS, LEAH; Xiao, Rong; Liu, Jinfeng; BARAN, MICHAEL; SWAPNA, G.V.T; ACTON, THOMAS; Rost, BURKHARD; MONTELIONE, GAETANO

Citation: Liu, Gaohua; MONTELIONE, Gaetano. "SOLUTION STRUCTURE OF 30S RIBOSOMAL PROTEIN S8E FROM Methanothermobacter thermautotrophicus, NORTHEASTSTRUCTURAL GENOMICS CONSORTIUM (NESG) TARGET"  Not known ., .-..

Assembly members:
rps8e, polymer, 294 residues, 33088.738 Da.

Natural source:   Common Name: Methanobacterium thermoautotrophicum   Taxonomy ID: 145262   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanobacterium thermoautotrophicum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
rps8e: GDRRVRLIRTRGGNTKVRLA SDTRINVVDPETGKVEIAEI RNVVENTANPHFVRRNIITR GAVVETNLGNVRVTSRPGQD GVINGVLIRELEHHHHHHGD RRVRLIRTRGGNTKVRLASD TRINVVDPETGKVEIAEIRN VVENTANPHFVRRNIITRGA VVETNLGNVRVTSRPGQDGV INGVLIRELEHHHHHHGDRR VRLIRTRGGNTKVRLASDTR INVVDPETGKVEIAEIRNVV ENTANPHFVRRNIITRGAVV ETNLGNVRVTSRPGQDGVIN GVLIRELEHHHHHH

Data sets:
Data typeCount
13C chemical shifts303
15N chemical shifts97
1H chemical shifts662

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rps8e1

Entities:

Entity 1, rps8e 294 residues - 33088.738 Da.

1   GLYASPARGARGVALARGLEUILEARGTHR
2   ARGGLYGLYASNTHRLYSVALARGLEUALA
3   SERASPTHRARGILEASNVALVALASPPRO
4   GLUTHRGLYLYSVALGLUILEALAGLUILE
5   ARGASNVALVALGLUASNTHRALAASNPRO
6   HISPHEVALARGARGASNILEILETHRARG
7   GLYALAVALVALGLUTHRASNLEUGLYASN
8   VALARGVALTHRSERARGPROGLYGLNASP
9   GLYVALILEASNGLYVALLEUILEARGGLU
10   LEUGLUHISHISHISHISHISHISGLYASP
11   ARGARGVALARGLEUILEARGTHRARGGLY
12   GLYASNTHRLYSVALARGLEUALASERASP
13   THRARGILEASNVALVALASPPROGLUTHR
14   GLYLYSVALGLUILEALAGLUILEARGASN
15   VALVALGLUASNTHRALAASNPROHISPHE
16   VALARGARGASNILEILETHRARGGLYALA
17   VALVALGLUTHRASNLEUGLYASNVALARG
18   VALTHRSERARGPROGLYGLNASPGLYVAL
19   ILEASNGLYVALLEUILEARGGLULEUGLU
20   HISHISHISHISHISHISGLYASPARGARG
21   VALARGLEUILEARGTHRARGGLYGLYASN
22   THRLYSVALARGLEUALASERASPTHRARG
23   ILEASNVALVALASPPROGLUTHRGLYLYS
24   VALGLUILEALAGLUILEARGASNVALVAL
25   GLUASNTHRALAASNPROHISPHEVALARG
26   ARGASNILEILETHRARGGLYALAVALVAL
27   GLUTHRASNLEUGLYASNVALARGVALTHR
28   SERARGPROGLYGLNASPGLYVALILEASN
29   GLYVALLEUILEARGGLULEUGLUHISHIS
30   HISHISHISHIS

Samples:

sample_1: rps8e, [U-100% 13C; U-100% 15N], 0.94 mM; H2O 95%; D2O 5%

sample_2: rps8e, [U-10% 13C; U-100% 15N], 1 mM; H2O 95%; D2O 5%

sample_1d20: rps8e, [U-100% 13C; U-100% 15N], 0.94 mM; D2O 100%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1d20isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1d20isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - geometry optimization, refinement, structure solution

AutoStruct, Huang, Tejero, Powers and Montelione - geometry optimization, peak picking, refinement

PSVS, Bhattacharya and Montelione - geometry optimization

AutoAssign, Zimmerman, Moseley, Kulikowski and Montelione - chemical shift assignment

XEASY, Bartels et al. - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

VNMRJ, Varian - collection

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 800 MHz
  • Varian INOVA 600 MHz

Related Database Links:

BMRB 16820