BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16069

Title: Resonance assignments for PilZ from Xanthomonas citri   PubMed: 19646999

Authors: Guzzo, Cristiane; Salinas, Roberto; Farah, Chuck

Citation: Guzzo, Cristiane; Salinas, Roberto; Andrade, Maxuel; Farah, Chuck. "PILZ protein structure and interactions with PILB and the FIMX EAL domain: implications for control of type IV pilus biogenesis."  J. Mol. Biol. 393, 848-866 (2009).

Assembly members:
PilZ, polymer, 117 residues, Formula weight is not available

Natural source:   Common Name: Xanthomonas axonopodis   Taxonomy ID: 53413   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Xanthomonas axonopodis

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PilZ: MSAMNARQGILSLALKDKPA LYSAYMPFVKGGGIFVPTPK RYMLGDEVFLLLTLPDSSER LPVAGKVIWTTPAGAQGNRA AGIGVQFPDGPEGEAVRNKI ETLLAGLTTSDKPTHTM

Data sets:
Data typeCount
13C chemical shifts184
15N chemical shifts93
1H chemical shifts93
heteronuclear NOE values87

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PilZ1

Entities:

Entity 1, PilZ 117 residues - Formula weight is not available

1   METSERALAMETASNALAARGGLNGLYILE
2   LEUSERLEUALALEULYSASPLYSPROALA
3   LEUTYRSERALATYRMETPROPHEVALLYS
4   GLYGLYGLYILEPHEVALPROTHRPROLYS
5   ARGTYRMETLEUGLYASPGLUVALPHELEU
6   LEULEUTHRLEUPROASPSERSERGLUARG
7   LEUPROVALALAGLYLYSVALILETRPTHR
8   THRPROALAGLYALAGLNGLYASNARGALA
9   ALAGLYILEGLYVALGLNPHEPROASPGLY
10   PROGLUGLYGLUALAVALARGASNLYSILE
11   GLUTHRLEULEUALAGLYLEUTHRTHRSER
12   ASPLYSPROTHRHISTHRMET

Samples:

sample_1: PilZ, [U-99% 13C; U-99% 15N], 0.5 mM; Tris buffer 5 mM; NaCl 150 mM

sample_2: PilZ, [U-99% 15N], 0.5 ± 0.05 mM; Tris buffer 5 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 0.150 M; pH: 7.0; pressure: 1 atm; temperature: 300 K

sample_conditions_2: ionic strength: 0.150 M; pH: 7.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
1H-15N NOEsample_2isotropicsample_conditions_2

Software:

Analysis_CCPN v1.0.14, CCPN - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker DRX 500 MHz

Related Database Links:

PDB
DBJ BAE67567 GAE49623 GAE57138 GAE61747
EMBL CAJ22783 CAP52699 CCF68085 CCG36105 CDF60728
GB AAM36006 AAM40324 AAW74141 AAY50265 ACD60951
REF NP_636400 WP_002814313 WP_003483889 WP_007969015 WP_011036225