BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15948

Title: 1H, 13C, and 15N Chemical Shift Assignments for the RING finger of the E3 ubiquitin ligase Arkadia (RNF111)   PubMed: 19032943

Authors: Kandias, Nikolaos; Chasapis, Christos; Episkopou, Vasso; Bentrop, Detlef; Spyroulias, Georgios

Citation: Kandias, Nikolaos; Chasapis, Christos; Bentrop, Detlef; Episkopou, Vasso; Spyroulias, Georgios. "High yield expression and NMR characterization of Arkadia E3 ubiquitin ligase RING-H2 finger domain"  Biochem. Biophys. Res. Commun. 378, 498-502 (2009).

Assembly members:
E3_ubiquitin_ligase_Arkadia, polymer, 69 residues, 7951.0 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
E3_ubiquitin_ligase_Arkadia: MKQDGEEGTEEDTEEKCTIC LSILEEGEDVRRLPCMHLFH QVCVDQWLITNKKCPICRVD IEAQLPSES

Data sets:
Data typeCount
13C chemical shifts290
15N chemical shifts71
1H chemical shifts467

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RING Finger1
2Zinc ion, 12
3Zinc ion, 22

Entities:

Entity 1, RING Finger 69 residues - 7951.0 Da.

1   METLYSGLNASPGLYGLUGLUGLYTHRGLU
2   GLUASPTHRGLUGLULYSCYSTHRILECYS
3   LEUSERILELEUGLUGLUGLYGLUASPVAL
4   ARGARGLEUPROCYSMETHISLEUPHEHIS
5   GLNVALCYSVALASPGLNTRPLEUILETHR
6   ASNLYSLYSCYSPROILECYSARGVALASP
7   ILEGLUALAGLNLEUPROSERGLUSER

Entity 2, Zinc ion, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: E3 ubiquitin ligase Arkadia, [U-98% 13C; U-98% 15N], 1.0 ± 0.2 mM; Pi 50 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.9; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
2D (HB)CB(CGCD)HDaromsample_1isotropicsample_conditions_1
2D 2J-edited 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAC04531 BAC29394 BAC33245 BAD18471 BAD18633
EMBL CAB75669 CAD98031 CAH93440
GB AAH10369 AAH20984 AAH54842 AAH60862 AAH69835
REF NP_001095709 NP_001100306 NP_001124565 NP_001186680 NP_001257457
SP Q5R476 Q6ZNA4 Q99ML9
TPG DAA25343