BMRB Entry 15875

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15875

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Title:
Backbone Chemical Shift Assignments and Relaxation Data for S. pombe Aps1d2-19
Deposition date:
2008-07-14
Original release date:
2009-05-29
Authors:
Garza, John; Ilangovan, Udayar; Hinck, Andrew; Barnes, Larry
Citation:

Citation: Garza, John; Ilangovan, Udayar; Hinck, Andrew; Barnes, Larry. "Kinetic, Dynamic, Ligand Binding Properties, and Structural Models of a Dual-Substrate Specific Nudix Hydrolase from Schizosaccharomyces pombe"  Biochemistry 48, 6224-6239 (2009).
PubMed: 19462967

Assembly members:

Assembly members:
Nudix_hydrolase, polymer, 216 residues, 24444 Da.

Natural source:

Natural source:   Common Name: fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nudix_hydrolase: MGSSHHHHHHHHHHSSGLVP RGSHMVNRSMTSREGRTKNR FNPITGARLAAGVVALSADK RKVLLVSSAKKHPSWVVPKG GWEADESVQQAALREGWEEG GLVGHITRSLGSFKDKRPTD TIDRRKKYLKQLMSKSSGND VSTNTELGAEAEKLLLPPRA ECEFFEVIVERLEDNYPEMR KRRRKWMSYQEAKEALTSRK DILAALEKSSIIKEEN

Data sets:
Data typeCount
13C chemical shifts503
15N chemical shifts159
1H chemical shifts159

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Nudix hydrolase1

Entities:

Entity 1, Nudix hydrolase 216 residues - 24444 Da.

Initial 24 residues, labeled (-23-0) are non-native His tag sequence. M is residue 1 and the following V is residue 20 then 21, 22 etc. These are labeled according to wildtype Aps1.

1   METGLYSERSERHISHISHISHISHISHIS
2   HISHISHISHISSERSERGLYLEUVALPRO
3   ARGGLYSERHISMETVALASNARGSERMET
4   THRSERARGGLUGLYARGTHRLYSASNARG
5   PHEASNPROILETHRGLYALAARGLEUALA
6   ALAGLYVALVALALALEUSERALAASPLYS
7   ARGLYSVALLEULEUVALSERSERALALYS
8   LYSHISPROSERTRPVALVALPROLYSGLY
9   GLYTRPGLUALAASPGLUSERVALGLNGLN
10   ALAALALEUARGGLUGLYTRPGLUGLUGLY
11   GLYLEUVALGLYHISILETHRARGSERLEU
12   GLYSERPHELYSASPLYSARGPROTHRASP
13   THRILEASPARGARGLYSLYSTYRLEULYS
14   GLNLEUMETSERLYSSERSERGLYASNASP
15   VALSERTHRASNTHRGLULEUGLYALAGLU
16   ALAGLULYSLEULEULEUPROPROARGALA
17   GLUCYSGLUPHEPHEGLUVALILEVALGLU
18   ARGLEUGLUASPASNTYRPROGLUMETARG
19   LYSARGARGARGLYSTRPMETSERTYRGLN
20   GLUALALYSGLUALALEUTHRSERARGLYS
21   ASPILELEUALAALALEUGLULYSSERSER
22   ILEILELYSGLUGLUASN

Samples:

sample_15N: Nudix_hydrolase, [U-99% 15N], 1.4 mM; H2O 95%; D2O, [U-100% 2H], 5%; sodium phosphate 25 mM

sample_13C-15N: Nudix_hydrolase, [U-99% 13C; U-99% 15N], 1.2 mM; H2O 95%; D2O, [U-100% 2H], 5%; sodium phosphate 25 mM

sample_2H-13C-15N: Nudix_hydrolase, [U-100% 13C; U-100% 15N; 80% 2H], 0.35 mM; H2O 95%; D2O, [U-100% 2H], 5%; sodium phosphate 25 mM

sample_15N-Lys: Nudix_hydrolase, [U-15N]-Lys, 1.4 mM; H2O 95%; D2O, [U-100% 2H], 5%; sodium phosphate 25 mM

sample_15N-Leu: Nudix_hydrolase, [U-15N]-Leu, 1 mM; H2O 95%; D2O, [U-100% 2H], 5%; sodium phosphate 25 mM

sample_conditions_1: ionic strength: 25 mM; pH: 6.4; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
3D C(CO)NHsample_13C-15Nisotropicsample_conditions_1
3D HNCAsample_2H-13C-15Nisotropicsample_conditions_1
3D HNCACBsample_2H-13C-15Nisotropicsample_conditions_1
3D HN(CO)CACBsample_2H-13C-15Nisotropicsample_conditions_1
3D HNCOsample_2H-13C-15Nisotropicsample_conditions_1
3D HN(CA)COsample_2H-13C-15Nisotropicsample_conditions_1
1H-15N heteronuclear NOEsample_15Nisotropicsample_conditions_1
15N T1sample_15Nisotropicsample_conditions_1
15N T2sample_15Nisotropicsample_conditions_1
2D 1H-15N HSQCsample_2H-13C-15Nisotropicsample_conditions_1
2D 1H-15N HSQCsample_15N-Lysisotropicsample_conditions_1
2D 1H-15N HSQCsample_15N-Leuisotropicsample_conditions_1

Software:

NMRView, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Johnson, One Moon Scientific - data analysis, data analysis, processing

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Related Database Links:

EMBL AAD20015 CAA91107
GB AAD20015
REF NP_592840
SP Q09790
AlphaFold Q09790

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks