BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15832

Title: chemical shift assignments and heteronuclear NOE of the N-terminal domain of the human Y5-receptor   PubMed: 19125388

Authors: Zerbe, Oliver; Walser, Reto; Zou, Chao; Kumaran, Sowmini

Citation: Zou, Chao; Kumaran, Sowmini; Walser, Reto; Zerbe, Oliver. "Properties of the N-terminal domains from Y receptors probed by NMR spectroscopy."  J. Pept. Sci. 15, 184-191 (2009).

Assembly members:
N-Y5, polymer, 51 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
N-Y5: MSFYSKQDYNMDLELDEYYN KTLATENNTAATRNSDFPVW DDYKSSVDDLQ

Data sets:
Data typeCount
15N chemical shifts48
1H chemical shifts48
heteronuclear NOE values47

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-Y51

Entities:

Entity 1, N-Y5 51 residues - Formula weight is not available

1   METSERPHETYRSERLYSGLNASPTYRASN
2   METASPLEUGLULEUASPGLUTYRTYRASN
3   LYSTHRLEUALATHRGLUASNASNTHRALA
4   ALATHRARGASNSERASPPHEPROVALTRP
5   ASPASPTYRLYSSERSERVALASPASPLEU
6   GLN

Samples:

sample_1: N-Y5, [U-15N], 0.5 mM; sodium phosphate 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_2: N-Y5, [U-15N], 0.5 mM; sodium phosphate 20 mM; DPC 300 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 310 K

sample_conditions_2: pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N NOEsample_2isotropicsample_conditions_2

Software:

XEASY, Bartels et al. - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz

Related Database Links:

DBJ BAG74150 BAK62588
GB AAC50623 AAC50741 AAC51295 AAG40773 AAH42416
REF NP_001028005 NP_001266807 NP_006165 XP_003899379 XP_004040628
SP Q15761